LC3、MMP-14及PD-1在喉癌中的表达及与病理特征和预后的相关性  

The expression of LC3,MMP-14 and PD-1 in laryngeal carcinoma and their correlation with pathological features and prognosis

作　　者：吴筱蔓 周兴星 顾翔 喻慧岭 潘晓玲 周敏[1] WU Xiaoman;ZHOU Xingxing;GU Xiang;YU Huiling;PAN Xiaoling;ZHOU Min(Wuhan Central Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430000, China)

机构地区：[1]华中科技大学附属武汉中心医院,湖北武汉430000

出　　处：《西部医学》2021年第12期1787-1792,共6页Medical Journal of West China

基　　金：武汉市医学科研项目(WX21Q51)。

摘　　要：目的探讨微管相关蛋白1轻链3(LC3)、基质金属蛋白酶-14(MMP-14)及程序性死亡因子1(PD-1)在喉癌中的表达及与病理特征、预后的相关性。方法回顾性分析于2016年7月~2018年7月我院收治的36例喉癌患者的临床资料,所有患者均经手术或穿刺活检证实,36份喉癌标本设为观察组,同时选取上述36例喉癌患者手术切除的正常组织作为对照组。分析LC3、MMP-14及PD-1在不同组织中的表达情况,探讨LC3、MMP-14及PD-1表达与病理特征及影响喉癌患者预后生存的因素。结果喉癌组织及正常组织中的LC3、MMP-14及PD-1阳性表达率比较差异有统计学意义(P<0.05)。LC3、MMP-14及PD-1的表达与临床分期、淋巴结转移、分化程度有关(P<0.05),LC3表达阴性及表达阳性中位生存期分别为(28.65±2.49)个月、(32.92±1.99)个月,MMP-14表达阴性及表达阳性中位生存期分别为(30.46±1.48)个月、(24.48±1.79)个月,PD-1表达阴性及表达阳性中位生存期分别为(25.71±2.04)个月、(19.40±1.63)个月。生存分析显示LC3表达阳性者生存期较长,MMP-14及PD-1表达阴性者生存期较长(P<0.05)。经非条件多因素Logistic回归模型进一步分析结果显示,临床分期(Ⅲ~Ⅵ期)、有淋巴结转移、高分化、LC3表达阳性、MMP-14表达阳性及PD-1表达阳性为影响喉癌患者预后生存的独立危险因素(P<0.05)。结论LC3阴性表达及MMP-14、PD-1阳性表达会增加喉癌患者死亡率,临床分期(Ⅲ~Ⅵ期)、有淋巴结转移、高分化、LC3表达阴性、MMP-14表达阳性及PD-1表达阳性为影响喉癌患者预后生存的独立危险因素;LC3、MMP-14、PD-1可作为判断喉癌患者肿瘤进展及预后的标志物。Objective To explore the expression of microtubule-associated protein 1 light chain 3(LC3),Recombinant Matrix Metalloproteinase 14(MMP-14),and mouse Programmed Death 1(PD-1)in laryngeal cancer and their correlation with pathological characteristics and prognosis.Methods The clinical data of 36 patients with laryngeal cancer admitted to our hospital from July 2016 to July 2018 were retrospectively analyzed.The expression of LC3,MMP-14 and PD-1 in different tissues was analyzed.The correlation between the expression of LC3,MMP-14 and PD-1 and pathological characteristics and the factors affecting the prognosis and survival of patients with laryngeal cancer were explored.Results There were significant differences in the positive expression rates of LC3,MMP-14 and PD-1 between laryngeal cancer tissues and normal tissues(P<0.05).The expression of the above indicators is related to the clinical stage,lymph node metastasis,and degree of differentiation(P<0.05).The average survival time of LC3 negative expression and positive expression was(28.65±2.49)months and(32.92±1.99)months,respectively.The average survival time of negative expression and positive expression of MMP-14 were(30.46±1.48)months and(24.48±1.79)months,respectively.The average survival period of negative expression and positive expression of PD-1 were(25.71±2.04)months and(19.40±1.63)months,respectively.Survival analysis showed that those with positive LC3 expression had a longer survival time,while those with negative MMP-14 and PD-1 expression had a longer survival time(P<0.05).Further analysis of the unconditional multivariate logistic regression model showed that clinical stage(stageⅢ~Ⅵ),lymph node metastasis,degree of differentiation(highly differentiated),LC3 expression(positive),MMP-14 expression(positive)and PD-1 expression(positive)were independent risk factors affecting the prognosis of patients with laryngeal cancer(P<0.05).Conclusion Negative expression of LC3 and positive expression of MMP-14 and PD-1 will increase the mortality of

关 键 词：LC3 MMP-14 PD-1 喉癌 病理特征 

分 类 号：R739.65[医药卫生—肿瘤]