非小细胞肺癌血清外泌体miR-152-5p和AGAP2-AS1水平变化及其预后评估价值  

Changes of serum exosomes miR-152-5p and AGAP2-AS1 levels and their evaluation value for prognosis in non-small cell lung cancer

作　　者：张旭 魏华华 王伟[1] 石俊青 张艺凡 ZHANG Xu;WEI Huahua;WANG Wei;SHI Junqing;ZHANG Yifan(The Second Ward, Department of Respiratory Medicine, Chengdu Hospital of Integrated Traditional Chinese and Western Medicine, Chengdu First People's Hospital, Chengdu 610041, China;Department of Respiratory Medicine, Chengdu Third People's Hospital, Chengdu 610000, China)

机构地区：[1]成都市中西医结合医院·成都市第一人民医院呼吸内科二病区,四川成都610041 [2]成都市第三人民医院呼吸内科,四川成都610000

出　　处：《西部医学》2021年第12期1844-1848,1853,共6页Medical Journal of West China

基　　金：四川省科技厅攻关项目(0040205301469)。

摘　　要：目的探讨非小细胞肺癌(NSCLC)血清外泌体miR-152-5p和AGAP2-AS1水平变化及其预后评估价值。方法选取成都市第一人民医院2014年6月~2016年12月收治的120例NSCLC患者临床资料(NSCLC组),另选择60例同期于本院进行体检的健康人群作为对照组。分离并鉴定血清外泌体,通过RT-PCR检测miR-152-5p和lncRNA AGAP2-AS1表达水平,分析NSCLC患者血清外泌体miR-152-5p和lncRNA AGAP2-AS1水平变化,探讨其与临床病理特征的关系。依照NSCLC患者随访3年预后情况,将患者分为生存组与死亡组,分析血清外泌体miR-152-5p和lncRNA AGAP2-AS1在非小细胞肺癌中的诊断及预后评估价值。结果NSCLC组患者miR-152-5p表达水平低于对照组,lncRNA AGAP2-AS1表达水平高于对照组(P<0.05);miR-152-5p、lncRNA AGAP2-AS1联合检测诊断NSCLC的发生ROC曲线下面积高于单独检测ROC曲线下面积(P<0.05);不同性别、年龄NSCLC患者miR-152-5p、lncRNA AGAP2-AS1表达水平比较差异无统计学意义(P>0.05);临床分期Ⅲ~Ⅳ期、局部浸润T3~T4、发生淋巴结转移患者miR-152-5p表达低于Ⅰ~Ⅱ期、局部浸润T1/T2、无淋巴结转移患者,lncRNA AGAP2-AS1表达高于Ⅰ~Ⅱ期、局部浸润T1~T2、无淋巴结转移患者(P<0.05);miR-152-5p、lncRNA AGAP2-AS1联合检测判断NSCLC患者Ⅰ~Ⅱ期与Ⅲ~Ⅳ期ROC曲线下面积高于单独检测ROC曲线下面积(P<0.05)。随访3年,120例NSCLC患者中共有5例失访,其余115例患者中生存组患者66例,死亡组患者49例,生存组miR-152-5p表达量高于死亡组,lncRNA AGAP2-AS1表达量低于死亡组(P<0.05);miR-152-5p、lncRNA AGAP2-AS1联合检测判断NSCLC预后ROC曲线下面积高于单独检测ROC曲线下面积(P<0.05)。结论NSCLC患者血清外泌体miR-152-5p呈现低表达,lncRNA AGAP2-AS1呈现高表达,二者表达水平与NSCLC临床分期、肿瘤浸润、淋巴结转移有关,在患者疾病诊断与预后评估中具有重要价值。Objective To explore the changes in levels of serum exosomes miR-152-5p and AGAP2-AS1 in non-small cell lung cancer(NSCLC)and their evaluation value for prognosis.Methods The clinical data of 120 NSCLC patients who were admitted to the hospital from June 2014 to December 2016 were collected.Another 60 healthy people who underwent physical examination in the hospital during the same period were enrolled as control group.Serum exosomes were isolated and identified.The expression levels of miR-152-5p and lncRNA AGAP2-AS1 were detected by RT-PCR.The changes in levels of serum exosomes miR-152-5p and lncRNA AGAP2-AS1 were analyzed.And their relationship with clinicopathology was explored.According to the prognosis after 3 years of follow-up,patients were divided into survival group and death group.The value of serum exosomes miR-152-5p and lncRNA AGAP2-AS1 in diagnosis of NSCLC and evaluation of prognosis was analyzed.Results The expression level of miR-152-5p in NSCLC group was lower than that in control group,while expression level of lncRNA AGAP2-AS1 was higher than that in control group(P<0.05).The area under the ROC curve(AUC)of miR-152-5p combined with lncRNA AGAP2-AS1 for diagnosis of NSCLC was 0.902,greater than that of miR-152-5p and lncRNA AGAP2-AS1 alone(0.813,0.808)(P<0.05).There were no significant differences in the expression levels of miR-152-5p and lncRNA AGAP2-AS1 among NSCLC patients with different genders and age(P>0.05).The expression level of miR-152-5p in patients with clinical staging at stageⅢ~Ⅳ,local invasion of T3~T4 and lymph node metastasis was lower than that with clinical staging at stageⅠ~Ⅱ,local invasion of T1~T2 and without lymph node metastasis,while expression level of lncRNA AGAP2-AS1 was higher than that in these kinds of patients(P<0.05).AUC of miR-152-5p combined with lncRNA AGAP2-AS1 for determining stageⅠ~ⅡandⅢ~Ⅳwas 0.902,higher than that of them alone(0.802,0.815)(P<0.05).After 3 years of follow-up,there were 5 cases lost to follow-up among the 120 NSCLC patie

关 键 词：非小细胞肺癌 血清外泌体 miR-152-5p AGAP2-AS1 预后 评估 

分 类 号：R734.2[医药卫生—肿瘤]