甲基转移酶3基因对前列腺癌细胞c-Myc、EGFR、Bcl-2表达的影响及其机制  

Effect of METTL3 gene on expression of c-Myc,EGFR and Bcl-2 in prostate cancer cells and its mechanism

作　　者：李显永 赵晖[2] 梁勇[1] 程阳[1] 韩登俊[1] 官润云[2] LI Xianyong;ZHAO Hui;LIANG Yong;CHENG Yang;HAN Dengjun;GUAN Runyun(Department of Urology,Zigong Fourth People's Hospital,Zigong 643000,China;不详)

机构地区：[1]自贡市第四人民医院泌尿外科,四川自贡643000 [2]昆明医科大学第一附属医院泌尿外科

出　　处：《山东医药》2021年第32期20-23,共4页Shandong Medical Journal

基　　金：云南省科技厅—昆明医科大学应用基础研究联合专项资金项目(2017FE467-042)。

摘　　要：目的观察甲基转移酶3(METTL3)基因对前列腺癌细胞系PC-3、DU145中肿瘤相关蛋白c-Myc、EGFR、Bcl-2表达的影响,并探讨相关作用机制。方法运用RNA干扰技术合成METTL3 siRNA-1400、阴性siRNA。将PC-3、DU145细胞分别分为空白对照组、阴性对照组、实验组,空白对照组不做特殊处理,阴性对照组转染阴性对照siRNA,实验组转染METTL3 siRNA-1400。采用Western blotting法检测各组细胞中的肿瘤相关蛋白c-Myc、EGFR、Bcl-2,Dolt blotting法检测各组细胞RNA的N6-甲基腺苷(m6A)修饰水平。结果PC-3细胞、DU145细胞的实验组c-Myc、EGFR、Bcl-2蛋白表达均低于空白对照组、阴性对照组(P均<0.01)。PC-3细胞、DU145细胞的实验组RNA m6A修饰水平均低于空白对照组和阴性对照组(P均<0.01)。结论沉默前列腺癌PC-3、DU145细胞中METTL3基因可抑制肿瘤相关蛋白c-Myc、EGFR、Bcl-2的表达,其机制可能与METTL3参与RNA的m6A修饰有关。Objective To observe the effects of methyltransferase-like 3(METTL3)gene on the expression levels of tumor proteins c-Myc,EGFR,and Bcl-2 in the prostate cancer cell lines PC-3 and DU145 and to investigate the mechanism.Methods We used RNA interference technology to synthesize METTL3 siRNA-1400 and negative siRNA.We divided PC-3 cells into the blank control group,negative control group,and experimental group,and also divided the DU145 cells into the blank control group,negative control group,and experimental group.The cells in the blank control group were not treated,the cells in the negative control group were transfected with negative control siRNA,and the cells in the experimental group were transfected with METL3 siRNA-1400,respectively.Western blotting was used to detect the expression of tumor proteins c-Myc,EGFR and Bcl-2 in each group.The m6A modification level of RNA in each group was detected by Dolt blotting.Results Western blotting showed that the protein levels of c-Myc,EGFR and Bcl-2 in the experimental groups were significantly lower than those in the blank control groups and negative control groups in the PC-3 and DU145 cells(all P<0.01).The m6A modification levels of RNA in the experimental groups were significantly lower than those in the blank control groups and negative control groups in the PC-3 and DU145 cells(all P<0.01).Conclusion Silencing METTL3 gene in prostate cancer PC-3 and DU145 cells inhibits the expression of c-Myc,EGFR and Bcl-2,and the underlying mechanism may be related to the involvement of METTL3 in m6A modification of RNA.

关 键 词：前列腺癌 甲基转移酶3 m6A修饰 C-MYC蛋白 EGFR蛋白 BCL-2蛋白 

分 类 号：R737.2[医药卫生—肿瘤]