维生素D受体基因多态性与妊娠期糖尿病的关系  

Association of polymorphisms in vitamin D receptor gene with gestational diabetes mellitus

作　　者：陈慧敏 萧敏华 孙静[1] 吴锦晖 罗红凤 刘喜红 CHEN Huimin;XIAO Minhua;SUN Jing;WU Jinhui;LUO Hongfeng;LIU Xihong(Department of Clinical Nutrition,Guangzhou Women and Children′s Medical Center,Guangzhou Medical University,Guangzhou 510623,China)

机构地区：[1]广州医科大学广州市妇女儿童医疗中心,广州510623

出　　处：《实用医学杂志》2021年第22期2866-2870,共5页The Journal of Practical Medicine

基　　金：广东省科技计划项目基金(编号:2017ZC0389)。

摘　　要：目的研究维生素D受体基因多态性与妊娠期糖尿病(GDM)的关系,探讨GDM的遗传易感因素,为GDM的防治提供依据。方法按纳入标准筛选孕24~28周时确诊为GDM的孕妇555例为病例组,同时选取646例正常孕妇为对照组。通过荧光定量PCR的方法检测VDR基因rs2228570和rs739837位点的基因分型。应用多因素logistic回归模型分析VDR基因多态性与GDM风险的关系。结果 GDM组rs2228570位点GA基因型频率(52.43%)高于对照组(50.77%),携带GA基因型可使GDM发生风险增加2.80倍(OR=2.80,95%CI:1.06~7.40,P=0.038);GDM组AA基因型频率(29.01%)显著高于对照组(24.15%),携带AA基因型可使GDM发生风险增加3.98倍(OR=3.98,95%CI:1.23~12.86,P=0.021);GDM组rs2228570位点A等位基因频率显著高于对照组(55.23%vs. 49.54%,OR=1.72,95%CI:1.04~2.85,P=0.035)。rs2228570位点在显性模型下表现出与GDM有关(OR=3.07,95%CI:1.20~7.86,P=0.019)。rs739837位点的基因型和等位基因频率在病例和对照组间差异无统计学意义(P> 0.05)。多因素logistic回归分析显示,糖尿病家族史和rs2228570位点多态性是GDM的独立危险因素(P <0.05)。结论维生素D受体基因rs2228570多态性位点与GDM的发病有关联,提示维生素D受体基因可能是中国人群GDM的易感基因之一。Objective To investigate the relation of multiple single nucleotide polymorphisms(SNPs)of vitamin D receptor gene with gestational diabetes mellitus,in order touncover genetic susceptibility factors and provide a foundation for GDM prevention and treatment. Methods According to inclusion criteria,555 pregnant women diagnosed with GDM during 24 ~ 28 weeks were assigned to the case group,while 646 healthy pregnant women were assigned to the control group. Fluorescence quantitative PCR was used to genotype the VDR gene polymorphisms rs2228570 and rs739837.To investigate the relationship between polymorphisms in the VDR gene and GDM risk,multivariate logistic regression models were used. Results The frequency of GA genotype of rs2228570 in GDM group(52.43%)was higher than that in control group(50.77%),individuals with the GA genotype had a 2.80-fold increased risk of developing GDM(OR = 2.80,95%CI:1.06 ~ 7.40,P = 0.038);The frequency of AA genotype in GDM group(29.01%)was significantly higher than that in control group(24.15%),individuals with the AA genotype had a 3.98-fold increased risk of developing GDM(OR = 3.98,95%CI:1.23 ~ 12.86,P = 0.021). The frequency of A allele of rs2228570 in GDM group was significantly higher than that in control group(55.23% vs.49.54%,OR = 1.72,95%CI:1.04 ~ 2.85,P = 0.035). The rs2228570 were associated with GDM in a dominant model(OR = 3.07,95%CI:1.20 ~ 7.86,P = 0.019). There were no statistically significant differences in genotypes and allele frequency distribution of rs739837 between the case and control groups(P > 0.05). Multivariate logistic regression analysis showed that family history of diabetes and rs2228570 polymorphism were independent risk factors for GDM(P<0.05). Conclusions The rs2228570 loci of VDR gene is associated with the pathogenesis of GDM,indicating that the vitamin D receptor gene might be one of the susceptibility genes to GDM in the Chinese population.

关 键 词：受体 骨化三醇 多态性 单核苷酸 糖尿病 妊娠 

分 类 号：R153.1[医药卫生—营养与食品卫生学]