CEA阳性细胞系的鉴定及其荷瘤小鼠模型建立  

Identification of CEA-positive cell lines and establishment of their tumor-bearing mouse models

作　　者：吕开绩 张丽芳[2] 卢叶挺 王高卿[1] 周新华[1] LYU Kaiji;ZHANG Lifang;LU Yeting;WANG Gaoqing;ZHOU Xinhua(Ningbo Medical Center LiHuiLi Hospital,Ningbo 315100,Zhejiang,China;1nstitute of Molecular Virology and Immunology,Wenzhou Medical University,Wenzhou 325000,Zhejiang,China)

机构地区：[1]宁波市医疗中心李惠利医院,宁波315100 [2]温州医科大学分子病毒与免疫学研究所

出　　处：《现代实用医学》2021年第11期1408-1410,1417,I0001,共5页Modern Practical Medicine

基　　金：浙江省自然科学基金资助项目(LGF18H160030);宁波市自然科学基金(2019A610214)。

摘　　要：目的鉴定HT-29、MKN-45及Hela细胞系中CEA的阳性表达率,并分别建立3个细胞系对应的荷瘤小鼠肿瘤模型,分析其成瘤特性。方法采用RT-PCR、western blot检测HT-29、MKN-45及Hela细胞中的CEA表达情况,采用免疫荧光实验检测3株细胞系的CEA蛋白表达情况。以2×10^(7)/ml细胞浓度接种小鼠建立动物模型,观察荷瘤小鼠生长情况并记录肿瘤大小,对其成瘤特性进行分析。结果CEA蛋白在HT-29、MKN-45细胞中表达,而在Hela细胞中不表达,CEA蛋白主要存在于细胞的胞质中。MKN-45荷瘤小鼠的成瘤潜伏期为(6.3±1.5)d,HT-29成瘤潜伏期为(6.0±2.2)d,而Hela细胞的成瘤潜伏期为(10.0±1.6)d。MKN-45、HT-29细胞的成瘤时间明显短于Hela细胞。结论CEA在HT-29、MKN-45细胞中表达,而在Hela细胞中不表达。成功建立了3株细胞对应的荷瘤小鼠模型,为CEA阳性肿瘤的发病基础以及治疗提供了动物模型基础。Objective To identify the expression of carcinoembryonic antigen(CEA)in the three cell lines of HT-29,MKN-45 and Hela,and establish tumor models of tumor-bearing mice corresponding to each cell line respectively,and then analyzed their tumorigenic characteristics.Methods RT-PCR and Western Blot were used to verify the CEA expression in HT-29,MKN-45 and Hela cells.The CEA protein of the three cell lines was verified and localized by immunofluorescence experiments.Finally,tumor-bearing mice were inoculated at a cell concentration of 2×10^(7)/ml.The growth of the tumor-bearing mice was observed and the tumor size was recorded,and their tumor-forming characteristics were analyzed.Results CEA protein was expressed in HT-29 and MKN-45 cells,but not in Hela cells.CEA protein mainly existed in the cytoplasm of cells.All tumor-bearing mice in the experimental group survived and formed tumors.The tumorigenesis latency of MKN-45 tumor-bearing mice was(6.3±1.5)d,the tumorigenesis latency of HT-29 was(6.0±2.2)d,and the tumorigenesis of Hela cells The incubation period was(10.0±1.6)d.Conclusions CEA is expressed in HT-29 and MKN-45 cells,but not in Hela cells.The tumor-bearing mouse models corresponding to the three cell lines have been successfully established.It provides an animal model basis for the pathogenesis and treatment of CEA-positive tumors.

关 键 词：CEA 免疫荧光 Balb/c-nu裸小鼠 动物模型 

分 类 号：R-332[医药卫生]