植物乳杆菌AR307胞外多糖的物化性质的研究  

Physicochemical properties of exopolysaccharide from Lactobacillus plantarum AR307

作　　者：冯小婉 张汇 赖凤羲 艾连中 FENG Xiaowan;ZHANG Hui;LAI Fengxi;AI Lianzhong(Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)

机构地区：[1]上海理工大学医疗器械与食品学院,上海食品微生物工程技术研究中心,上海200093

出　　处：《工业微生物》2021年第6期1-8,共8页Industrial Microbiology

基　　金：上海市科技兴农项目(2019-02-08-00-07-F01152);上海食品微生物工程技术研究中心(19DZ2281100);国家杰出青年科学基金(32025029)。

摘　　要：本文对植物乳杆菌AR307(Lactobacillus plantarum AR307)发酵产生的两种(LPE-1和LPE-2)酸性胞外多糖(Exopolysaccharide,EPS)的物化性质进行研究。通过乌式黏度计测定LPE-1和LPE-2的固有黏度分别为3.32 dL/g和3.85 dL/g。热重分析表明LPE-1和LPE-2具有较高的降解温度,分别为285℃和290℃左右。扫描电镜观察到LPE-1固体粉末的表观形貌呈现分支化状链状结构,而LPE-2分子则是多孔网状结构。LPE-1和LPE-2在0.15 mol/L NaCl溶液中能有效消除粒径的聚集现象。两个酸性多糖都不具有螺旋结构,温度、金属离子、络合剂和解旋剂因素均对LPE-1和LPE-2的构象产生不同程度的影响。这一研究为植物乳杆菌AR307酸性多糖构效关系及应用提供了理论基础。The physicochemical properties of two groups(LPE-1 and LPE-2)of acidic exopolysaccharides(EPS)from Lactobacillus plantarum AR307 were studied.The intrinsic viscosities of LPE-1 and LPE-2 were 3.32 dL/g and 3.85 dL/g,respectively.Thermogravimetric analysis showed that LPE-1 and LPE-2 had higher degradation temperature,about 285℃and 290℃,respectively.Scanning electron microscopy displayed that LPE-1 had branched chain structure,while LPE-2 had porous network structure.LPE-1 and LPE-2 could effectively eliminate the aggregation of particle size in 0.15 mol/L NaCl solution.Congo red experiment showed that the two components did not have triple helix structure.The conformations of LPE-1 and LPE-2 were affected by temperature,metal ions,complexing agent and spin remover.This study provided a theoretical basis for the structure-activity relationship and application of acid polysaccharides from Lactobacillus plantarum AR307.

关 键 词：胞外多糖 物化性质 溶液构象 

分 类 号：R28[医药卫生—中药学]