四十八份青花菜品种SSR指纹图谱构建  

Construction of DNA fingerprint for 48 broccoli cultivars

作　　者：林朦婕 温慧萍[1] 肖建中[1] 郑强[1] LIN Mengjie;WEN Huiping;XIAO Jianzhong;ZHENG Qiang(College of Ecology, Lishui University, Lishui 323000, China;College of Life Science, South China Normal University, Guangzhou 510631, China)

机构地区：[1]丽水学院生态学院,浙江丽水323000 [2]华南师范大学生命科学学院,广东广州510631

出　　处：《浙江农业学报》2021年第12期2304-2312,共9页Acta Agriculturae Zhejiangensis

基　　金：丽水市科技计划(2017GYX01)。

摘　　要：为筛选一套适用于青花菜品种DNA指纹鉴定的核心简单重复序列标记(simple sequence repeat,SSR)引物,给青花菜品种的特异性、真实性、准确性鉴定提供依据,以12份性状差异较大的青花菜品种为材料,结合聚丙烯酰胺凝胶电泳与荧光毛细管电泳检测技术,从945对芸薹属SSR引物中筛选出20对引物作为青花菜品种的核心SSR引物。利用该套核心SSR引物在48份青花菜主要栽培品种中共扩增出等位基因位点79个,平均每对引物扩增得到等位基因与基因型数量为3.95、5.55个;引物多态性信息含量(PIC)介于0.302~0.750,平均为0.547;20对核心SSR引物的杂合度介于0.3500~0.7849,均值为0.6082。用该套核心SSR引物构建的48份青花菜品种的指纹图谱,每一条指纹都具有唯一性,可表征一个品种,该指纹图谱为青花菜品种鉴定与资源保护提供了科学依据。To screen a set of core SSR(simple sequence repeat)primers for identifying of broccoli cultivars,12 genetically different broccoli varieties were chosen as screening materials,combined polyacrylamide gel electrophoresis and fluorescence capillary electrophoresis techniques,20 pairs of core SSR primers were finally selected from a total of 945 pairs of Brassica SSR primers.Using these 20 pairs of core primers,79 polymorphic alleles were amplified from 48 common broccoli cultivars,with 3.95 polymorphic alleles and 5.55 genotypes per pair of primer in average.The polymorphism information content(PIC)value ranged from 0.302 to 0.750,with an average of 0.547,and the heterozygosity ranged from 0.3500 to 0.7849,with an average value of 0.6082.Each of the fingerprints of 48 broccoli cultivars constructed with the core SSR primers was unique and could be used to characterize one cultivar,which provided a scientific basis for broccoli variety identification and resource protection.

关 键 词：青花菜 简单重复序列标记 指纹图谱 等位基因 多态性信息含量 

分 类 号：S635[农业科学—蔬菜学]