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作 者:陈润臣 王艺凝 刘潇文 王红艳[1] 丁强[1] 王鸿磊[1] CHEN Runchen;WANG Yining;LIU Xiaowen;WANG Hongyan;DING Qiang;WANG Honglei(Yantai Institute of China Agricultural University, Yantai 264670, China)
机构地区:[1]中国农业大学烟台研究院,山东烟台264670
出 处:《浙江农业学报》2021年第12期2330-2338,共9页Acta Agriculturae Zhejiangensis
基 金:山东省农业重大应用技术创新课题;山东省农业科学院科技创新重点项目(2014CXZ02-2);山东省产业体系食用菌烟台综合试验站(SDAIT-07-14);中国农业大学烟台研究院校级课题(YT201901,YT201904)。
摘 要:为丰富食用菌栽培品种,采用形态学和分子生物学方法对一株野生鳞伞属真菌进行了鉴定,研究了菌丝的生物学特性,进行了人工栽培试验并测定了子实体的营养成分。结果显示:该鳞伞属真菌为多脂鳞伞;菌丝最适生长温度为25℃,最适生长pH值为5.0,最佳碳源、氮源和无机盐分别是蔗糖、豆饼粉和MnSO_(4);通过人工栽培成功获得子实体,从接种到采收约需70 d;子实体蛋白质含量为19.94%,多糖含量为5.14%,脂肪含量为2.32%,游离氨基酸含量为2.84%。In order to enrich the cultivated varieties of edible fungi,a wild fungus was identified by morphological and molecular biological methods,biological characteristics of hyphae were studied,artificial cultivation experiments were carried out,and nutritional components of fruiting bodies were determined.The results showed that the fungus was identified as Pholiota adiposa.The optimal growth temperature was 25℃,the optimal pH value was 5.0,and the optimal carbon source,nitrogen source and inorganic salt were sucrose,soybean cake powder and MnSO_(4),respectively.Fruiting bodies were successfully cultivated from agricultural wastes such as sawdust and corncob,it took about 70 days from inoculation to harvest.Protein content,polysaccharide content,fat content and free amino acid content of fruit body were 19.94%,5.14%,2.32%and 2.84%,respectively.
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