胃癌患者SFRP1、SFRP2、SDC-2甲基化与病理特征的相关性分析  

Correlation analysis of SFRP1,SFRP2 and SDC-2 methylation and pathological features in patients with gastric cancer

作　　者：黄一波 王国平[1] 王奕 莫文魁 HUANG Yi-bo;WANG Guo-ping;WANG Yi;MO Wen-kui(Department of General Surgery,the Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine,Hangzhou,Zhejiang 310005,China)

机构地区：[1]浙江中医药大学附属二院普外科,浙江杭州310005

出　　处：《中华全科医学》2021年第12期2008-2011,共4页Chinese Journal of General Practice

基　　金：浙江省医药卫生科技计划项目(2017KY517)。

摘　　要：目的分析胃癌患者分泌型卷曲相关蛋白1(SFRP1)、SFRP2及多能体蛋白聚糖-2(SDC-2)基因甲基化与胃癌临床病理特征的相关性。方法收集浙江中医药大学附属二院在2017年5月—2019年4月收治的接受胃癌根治术的100例患者,检测胃癌组织中SFRP1、SFRP2及SDC-2基因甲基化状态;分析上述基因甲基化率在不同临床病理特征的胃癌组织中的差异。结果癌肿组织中SDC-2、SFRP1、SFRP2基因甲基化率、肿瘤直径、浸润深度、淋巴结转移及分化程度存在不同程度的差异(均P<0.05);多因素logistic回归分析显示,SDC-2甲基化(OR=0.384)是影响癌肿大小的独立保护因素(P<0.05);SFRP2甲基化(OR=5.991)是影响肿瘤浸润深度的独立危险因素,SDC-2甲基化(OR=0.171)是影响肿瘤浸润深度的独立保护因素(均P<0.05);SFRP1甲基化(OR=4.614)是影响肿瘤淋巴结转移的独立危险因素,SDC-2甲基化(OR=0.345)是抑制肿瘤淋巴结转移的独立保护性因素(均P<0.05);SFRP1甲基化(OR=11.667)、SFRP2甲基化(OR=23.333)是影响肿瘤分化程度的独立危险因素(均P<0.05)。结论SFRP1、SFRP2及SDC-2基因甲基化状态与胃癌患者临床病理特征存在高度相关性,SFRP1、SFRP2基因的甲基化可能是促进肿瘤增长、迁移、低分化的相关因素。Objective To analyse the correlation between secreted frizzled related protein 1(SFRP1),SFRP2 and SDC-2 gene methylation and clinicopathological characteristics of gastric cancer.Methods A total of 100 patients who underwent radical gastric cancer surgery from May 2017 to April 2019 in the Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine were collected,and the methylation status of SFRP1,SFRP2 and SDC-2 genes in gastric cancer tissues was detected.The difference of the above-mentioned gene methylation rate in gastric cancer tissues with different clinicopathological characteristics was analysed.Results The methylation rates of SDC-2,SFRP1 and SFRP2 genes,tumor diameter,depth of invasion,lymph node metastasis and differentiation were different in cancer tissues(all P<0.05).Multivariate logistic regression analysis showed that SDC-2 methylation(OR=0.384)was an independent protective factor affecting cancer size(P<0.05).SFRP2 methylation(OR=5.991)was an independent risk factor affecting the depth of tumour invasion,whilst SDC-2 methylation(OR=0.171)was an independent protective factor affecting the depth of tumour invasion(all P<0.05).SFRP1 methylation(OR=4.614)was an independent risk factor affecting tumour lymph node metastasis,whilst SDC-2 methylation(OR=0.345)was an independent protective factor inhibiting tumour lymph node metastasis(all P<0.05).SFRP1 methylation(OR=11.667)and SFRP2 methylation(OR=23.333)were independent risk factors affecting tumour differentiation(all P<0.05).Conclusion The methylation status of SFRP1,SFRP2 and SDC-2 genes is highly correlated with the clinicopathological characteristics of gastric cancer patients.The methylation of SFRP1 and SFRP2 genes may be related factors that promote tumour growth,migration and poor differentiation.

关 键 词：胃癌 分泌型卷曲相关蛋白基因 甲基化 病理特征 

分 类 号：R735.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]