上调miR-1322通过靶向TRPV4对胃癌SGC7901细胞凋亡和迁移的影响  

Effect of up-regulation of miR-1322 on apoptosis and migration of gastric cancer SGC7901 cells by targeting TRPV4

作　　者：朱钟钟 姜进平[1] 黄耿[2] 罗海平[1] 汤守元[1] Zhu Zhongzhong;Jiang Jinping;Huang Geng;Luo Haiping;Tang Shouyuan(Department of Gastroenterology and Anorectal Surgery,Affiliated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Huangshi 435000,China;Department of Urology,Huangshi Central Hospital,Affiliated Hospital of Hubei Polytechnic University,Edong Healthcare Group,Huangshi 435000,China)

机构地区：[1]鄂东医疗集团黄石市中心医院湖北理工学院附属医院胃肠肛肠外科,黄石435000 [2]鄂东医疗集团黄石市中心医院湖北理工学院附属医院泌尿外科,黄石435000

出　　处：《国际医药卫生导报》2021年第23期3618-3621,共4页International Medicine and Health Guidance News

基　　金：湖北省卫生健康科研基金资助项目(WJ2019H158)。

摘　　要：目的探讨上调微小RNA(microRNA)-1322(miR-1322)对胃癌SGC7901细胞凋亡及迁移的影响,分析其作用机制。方法于2020年8月至2021年6月构建miR-1322慢病毒载体,感染胃癌SGC7901细胞为miR-1322组,感染空白慢病毒载体为对照组。实时定量聚合酶链式反应(qRT-PCR)分析SGC7901中miR-1322的表达。流式细胞术和Transwell实验检测上调miR-1322对SGC7901细胞凋亡和迁移的影响。生物信息学预测miR-1322的靶基因。qRT-PCR和Westernblot检测上调miR-1322对靶基因表达的影响。结果对照组和miR-1322组的miR-1322表达分别为(1.01±0.07)和(12.96±1.05),miR-1322组miR-1322表达是对照组的12.83倍(t=6.30,P<0.01)。对照组和miR-1322组SGC7901细胞凋亡比例分别为(6.95±1.02)%和(32.88±6.22)%,SGC7901细胞迁移数分别为(107.30±10.94)个和(43.44±8.04)个,与对照组相比,上调miR-1322可显著促进SGC7901细胞的凋亡率(t=4.11,P<0.01),抑制SGC7901细胞的迁移能力(t=4.28,P<0.01)。生物信息学显示瞬时受体电位香草酸4(TRPV4)可能是miR-1322的靶基因。与对照组比较,上调miR-1322可显著抑制SGC7901细胞中TRPV4基因的表达(P<0.01)。结论miR-1322可能通过靶向调控TRPV4基因表达,促进胃癌SGC7901细胞的凋亡并抑制其迁移。Objective To explore the effect of up-regulated microRNA-1322(miR-1322)on the apoptosis and migration of gastric cancer SGC7901 cell line,and to analyze its mechanism.Methods This study wsa from August 2020 to June 2021.the miR-1322 lentiviral vector was constructed.The gastric cancer SGC7901 cells infected with miR-1322 lentiviral vectors were used as an experimental group,and those infected with blank lentiviral vectors as a control group.Real-time quantitative polymerase chain reaction(qRT-PCR)was used to analyze the expression of miR-1322 in the SGC7901 cells.Flow cytometry and Transwell experiment were used to detect the effect of the up-regulation of miR-1322 on the apoptosis and migration of the SGC7901 cells.Bioinformatics was used to predict the target gene of miR-1322.qRT-PCR and Western blot were used to detect the effect of the up-regulated miR-1322 on the target gene expression.Results The expressions of miR-1322 in the control group and the miR-1322 group were(1.01±0.07)and(12.96±1.05),respectively.The expression of miR-1322 in the miR-1322 group was 12.83 times that of the control group(t=6.30,P<0.01).The apoptosis rates and the migration numbers of the SGC7901 cells in the control group and the miR-1322 group were(6.95±1.02)%,(32.88±6.22)%,(107.30±10.94),and(43.44±8.04),respectively,indicating that the up-regulation of miR-1322 could significantly promote the apoptosis rate(t=4.11,P<0.01)of and inhibit the migration ability of the SGC7901 cells(t=4.28,P<0.01).Bioinformatics showed that transient receptor potential vanilloid 4(TRPV4)might be the target gene of miR-1322.Compared with the control group,the up-regulation of miR-1322 could significantly inhibit the expression of TRPV4 gene in the SGC7901 cells(P<0.01).Conclusion miR-1322 may promote the apoptosis of and inhibit the migration ability of gastric cancer SGC7901 cells through the targeted regulation of TRPV4 gene expression.

关 键 词：胃癌 miR-1322 瞬时受体电位香草酸4 SGC7901 凋亡 迁移 

分 类 号：R735.2[医药卫生—肿瘤]