环氧丙烷对仿刺参抗氧化酶和免疫酶活性的影响  

Effects of Propylene Epoxide on Activities of Antioxidant and Immune Related Enzymes in Sea Cucumber Apostichopus japonicus

作　　者：王丽梅[1] 李多慧 罗耀明 鹿志创[1] 何平[1] WANG Limei;LI Duohui;LUO Yaoming;LU Zhichuang;HE Ping(Liaoning Key Laboratory of Marine Biological Resources and Ecology,Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China;Dalian Modern Agricultural Production Development Service Center,Dalian 116085,China)

机构地区：[1]辽宁省海洋水产科学研究院,辽宁省海洋生物资源和生态学重点实验室,辽宁大连116023 [2]大连市现代农业生产发展服务中心,辽宁大连116085

出　　处：《水产学杂志》2021年第6期59-64,共6页Chinese Journal of Fisheries

基　　金：辽宁省海洋与渔业厅科研项目(201832);辽宁省自然科学基金指导计划(20170540482).

摘　　要：将经暂养的体质量(5.23±0.96)g的仿刺参(Apostichopus japonicus)置于10 L玻璃缸中,水中环氧丙烷质量浓度为100.00 mg/L、200.00 mg/L、300.00 mg/L、400.00 mg/L、500.00 mg/L及600.00 mg/L(用母液调配),每个梯度设3个平行,每个缸中20头幼参,统计24 h、48 h、72 h、96 h幼参死亡率,同时测定体腔液中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、酸性磷酸酶(ACP)和碱性磷酸酶(ALP)的活性。试验结果表明,高质量浓度组仿刺参体腔液中抗氧化酶活性最先在24 h时、随后ALP活性在48 h时均受到显著诱导(P<0.05);48 h时各质量浓度组CAT活性最先极受到显著抑制(P<0.01),其他3种酶活性在72 h时开始受到显著抑制,72 h时CAT活性恢复,而高质量浓度组直到96 h,其他3种酶活性仍受到显著抑制(P<0.05)。CAT活性受到污染物的影响最大,但恢复也最快。除ACP外,其他3种酶活性均表现短期毒物兴奋效应,受到显著诱导(P<0.05),长时间后活性均受到显著抑制(P<0.05)。在环氧丙烷胁迫下仿刺参体腔液中的抗氧化酶活性受到影响时间比免疫酶短,受影响程度比免疫酶大。随着环氧丙烷质量浓度的增加和作用时间的延长,不同程度的影响仿刺参体腔液中4种酶的活性。环氧丙烷使仿刺参致毒的机制可能是产生自由基的氧化胁迫,影响消化、吸收和代谢。本研究结果可为研究环氧丙烷对海洋生物的毒理机制提供参考。Juvenile sea cucumber Apostichopus japonicas with body weight of(5.23±0.96)g were held in a 10 L glass cylinder at a density of 20 individuals per glass cylinder and exposed to propylene epoxide,a toxic pollutant in marine environment,at concentrations of 100.00 mg/L,200.00 mg/L,300.00 mg/L,400.00 mg/L,500.00 and 600.00 mg/L.Activities of superoxide dismutase(SOD),catalase(CAT),acid phosphatase(ACP)and alkaline phosphatase(AKP)in the coelomic fluid of sea cucumber and mortality were determined in 24 h,48 h,72 h,and 96 h by static bioassay to investigate the effects of propylene epoxide on activities of antioxidant and immune related enzymes in sea cucumber.The results showed that the activities of antioxidant enzymes at 24 h and the activities of ALP at 48 h in the coelomic fluid of sea cucumber were significantly induced in the high concentration group(P<0.05).The activities of CAT were found to be first inhibited very significantly in each concentration group at 48 h(P<0.01)and to be recovered at 72 h,and the activities of the other three enzymes were significantly inhibited at 72 h until 96 h(P<0.05),showing most significant influence by pollutants and the most rapid recovery.The activities of the other three enzymes were significantly induced in a short time(P<0.05)and significantly inhibited in a long time(P<0.05)by propylene epoxide except for ACP.Under the stress of propylene epoxide,the activities of antioxidant enzymes were affected in the coelomic fluid of sea cucumber.enzymes in the coelomic fluid of sea cucumber were found to be elevated with the increase inpropylene epoxide concentrations and time prolonged,indicating that the toxic mechanisms of propylene epoxide was involved in generated free radicals,oxidative stress and the effects on digestive and metabolic systems in sea cucumber.The findings provided the reference with ecological toxicology of propylene epoxide in marine organisms.

关 键 词：环氧丙烷 仿刺参 超氧化物歧化酶 过氧化氢酶 酸性磷酸酶 碱性磷酸酶 

分 类 号：X171.5[环境科学与工程—环境科学]