CdTe水溶性量子点的合成表征及生物毒性测定——一个化学生物学实验  

Synthesis and Characterization of CdTe Water-Soluble Quantum Dots and Its Bio-Toxicity Determination——A Chemical Biology Experiment

作　　者：刘成金[1] 陶海燕[1] 刘海燕 Liu Chengjin;Tao Haiyan;Liu Haiyan(College of Chemistry and Molecular Sciences,National Demonstration Center for Experimental Chemistry Kducation,Wuhan University,Wuhan,430072)

机构地区：[1]武汉大学化学与分子科学学院化学国家级实验教学示范中心,(武汉大学)武汉430072

出　　处：《化学通报》2021年第12期1383-1386,共4页Chemistry

基　　金：武汉大学实验技术项目(WHU-2020-XYKF-12)资助。

摘　　要：本实验以反应时间为变量,一步水相法合成了具有不同粒径的CdTe量子点。通过紫外-可见吸收光谱和荧光光谱表征不同反应时间得到的量子点,并计算其粒径、浓度以及荧光量子产率。取表征最优的量子点组次,配制成不同浓度,与酵母混合培养,用Biomass法测定CdTe量子点生物毒性。该实验涵盖无机合成相关知识,涉及分析表征方法应用以及量子点对生物细胞活性的影响,化学与生物学科交叉融合,有助于学生科研能力的培养与实验综合能力的提升。In this experiment,CdTe quantum dots were synthesized by one-step method in aqueous solution at different reacetion time.The ulraviolet-visible spectrum and fluorescenee speetra were used to characterize the obtained materials.According to the spectra.the diameter.concentration and fluorescence quantum yields of CdTe quantum dots can be caleulated.Then,the biological loxicily of CdTe quantum dots was determined by biomass method by mixing the different concentrations of CdTe quantum dots with yeast ells.This experiment covers inorganie synthesis.characterization and biochemistry.it's helpful for improving students’scientifie research ability and experimental comprehensive ability.

关 键 词：量子点 合成 荧光量子产率 生物毒性 化学生物学实验 

分 类 号：G642.423[文化科学—高等教育学] TB383.1-4[文化科学—教育学]