红托竹荪液体菌种培养基及培养条件优化  

Optimization for the Medium for Liquid Spawn and Culture Conditions of Dictyophora rubrovolvata

作　　者：卢征华 文庭池 徐彦军[3] 余刚江[1,2,3] 杨雨 LU Zhenghua;WEN Tingchi;XU Yanjun;YU Gangjiang;YANG Yu(College of Liquor and Food Engineering,Guizhou University,Guiyang,Guizhou 550025;The Engineering Research Center of Southwest Bio-Pharmaceutical Resources,Ministry of Education,Guizhou University,Guiyang,Guizhou 550025;Mushroom Research Institute,Guizhou University,Guiyang,Guizhou 550025)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州大学西南药用生物资源教育部工程研究中心,贵州贵阳550025 [3]贵州大学食用菌研究院,贵州贵阳550025

出　　处：《北方园艺》2021年第22期124-129,共6页Northern Horticulture

基　　金：贵州省科技基金资助项目(黔科合重大专项字[2019]3005-5号)。

摘　　要：以红托竹荪菌种为试材,采用液体摇瓶培养方法,研究了红托竹荪液体培养基的碳氮源筛选和培养条件优化,以期为红托竹荪液体菌种的制备和扩大培养提供参考依据。结果表明:最佳碳源为葡萄糖,最佳氮源为蛋白胨,最佳配方为葡萄糖30 g·L^(-1),蛋白胨1.5 g·L^(-1),KH_(2)PO_(4)2 g·L^(-1),MgSO41 g·L^(-1),(NH_(4))_(2)SO_(4)1 g·L^(-1),维生素C 0.016 g·L^(-1),生长素(IAA)0.0016 g·L^(-1)。最佳培养条件为25℃,摇床转速150 r·min^(-1),培养天数20 d。扩大培养的最佳培养条件为装液量每1000 mL装500 mL,接种量15%,培养天数15 d,从一级种制备到扩大培养共计35 d。Taking Dictyophora rubrovolvata as test material,the carbon and nitrogen source selection and culture condition optimization of its liquid medium of Dictyophora rubrovolvata were studied through liquid shake flask culture method,in order to provide a reference for the preparation and extended culture of liquid strains of Dictyophora rubrovolvata.The results showed that glucose and peptone were the optimal carbon source and nitrogen source,respectively.The optimal formula was 30 g·L^(-1) of glucose,1.5 g·L^(-1)of peptone,2 g·L^(-1)of KH_(2)PO_(4),1 g·L^(-1)of MgSO_(4),1 g·L^(-1) of(NH_(4))_(2)SO_(4),0.016 g·L^(-1) of vitamin C,and 0.0016 g·L^(-1) of indole acetic acid(IAA).Besides,the optimal culture conditions were 25℃,150 r·min^(-1) of shaking speed,and 20 days of culture period.The optimal culture conditions were as follows 500 mL of liquid per 1000 mL of culture volume,15%of inoculation size,and 15 days of culture period;35 days were required from first-degree species preparation to the extended culture.

关 键 词：红托竹荪 液体菌种 摇瓶培养 培养基优化 

分 类 号：S646.8[农业科学—蔬菜学]