黄金枸骨叶片复绿期叶绿素代谢的调控特征  

Regulation of Chlorophyll Metabolism during Leaf Greening Period of Ilex×attenuata‘Sunny Foster’

作　　者：黄婧[1] 苏金 周鹏[1] 张强[2] 张敏[1] Huang Jing;Su Jin;Zhou Peng;Zhang Qiang;Zhang Min(Jiangsu Academy of Forestry,Nanjing 211153,P.R.China;Nanjing Forestry University)

机构地区：[1]江苏省林业科学研究院,南京211153 [2]南京林业大学

出　　处：《东北林业大学学报》2021年第11期51-54,共4页Journal of Northeast Forestry University

基　　金：江苏省科技计划项目(BE2018400),江苏省林业科学研究院自主科研项目(BM2018022-6)。

摘　　要：为了探究黄金枸骨叶片变色期叶绿素代谢调控特征和叶片复绿期叶绿素合成中间产物的含量及关键酶活性。采用人工遮荫的方法,使黄金枸骨叶色由黄转绿,动态监测叶绿素的质量摩尔浓度、叶绿素合成前体物质的质量摩尔浓度、叶绿素合成酶活性和叶绿素降解酶活性的变化情况,分析叶片变绿过程中叶绿素生物合成代谢途径的特征。结果表明:叶片变色过程中叶绿素的质量摩尔浓度显著上升(P<0.05);δ-氨基乙酰丙酸(ALA)和胆色素原(PBG)的质量摩尔浓度显著下降(P<0.05),其后合成途径的中间产物尿卟啉原Ⅲ(Urogen Ⅲ)、粪卟啉原Ⅲ(Coprogen Ⅲ)、原卟啉Ⅸ(Proto Ⅸ)、Mg-原卟啉Ⅸ(Mg-Proto Ⅸ)、原叶绿素酸酯(Pchlide)的质量摩尔浓度显著增加(P<0.05);叶绿素合成相关酶,δ-氨基乙酰丙酸脱水酶活性显著下降(P<0.05),尿卟啉原Ⅲ合酶活性显著上升(P<0.05);叶绿素降解酶脱镁鳌合酶和叶绿素酶的活性显著下降(P<0.05)。因此,黄金构骨叶色由黄变绿期间,叶绿素合成酶活性升高,叶绿素前体物质的质量摩尔浓度显著增加,叶绿素的积累起始于尿卟啉原Ⅲ的增加,起始时间为遮荫后6~15 d,同时叶绿素降解酶活性下降,促进了叶绿素的积累,叶绿素的质量摩尔浓度升高,叶片由黄转绿。In order to explore the regulation of chlorophyll metabolism, especially the major precursors content and key enzymes activity of chlorophyll synthesis during discoloration period of Ilex×attenuata ’Sunny Foster’, the method of artificial shade is used to realize the leaf color from yellow to green. The molality of chlorophyll synthesis precursors, the activity of chlorophyll synthase and the activity of chlorophyll degradation enzymes were dynamically monitored, and the characteristics of chlorophyll biosynthetic pathways in the process of leaf greening were analyzed. The content of chlorophyll(Chl) increased sharply after shading(P<0.0.5). During the process of greening, the content of δ-aminolevulinic acid(ALA) and porphobilinogen(PBG) decreased significantly(P<0.0.5), the accumulation of uroporphyrinogen Ⅲ(Urogen Ⅲ), coproporphyrinogen Ⅲ(Coprogen Ⅲ), protoporphyrin Ⅸ(Proto Ⅸ), Mg-protoporphyrin Ⅸ(Mg-Proto Ⅸ) and protochlorophyllide(Pchlide) was increased significantly(P< 0.05). The activities of GluTR and ALA dehydratase decreased significantly(P<0.05), meanwhile the activity of Urogen Ⅲ synthase increased significantly(P<0.05), which are both chlorophyll synthesis-related enzymes. The activities of chlorophyll-degrading enzyme, demagnesium chelating enzyme and chlorophyllase activity both decreased significantly(P<0.05). As a conclusion, the greening of the leaves is mainly due to the increase of chlorophyll synthase activity, accumulation of chlorophyll synthesis precursors beginning with the increase of Urogen Ⅲ content after 6-15 d of shading, and the decrease in chlorophyll degrading enzyme activity, which together controlled the leaf color change and achieved leaf greening.

关 键 词：黄金枸骨 叶绿素 前体物质 酶活性 

分 类 号：Q945.11[生物学—植物学] S792.99[农业科学—林木遗传育种]