癌性爆发痛小鼠模型的优化与评价  

Optimization and evaluation of mouse model of breakthrough cancer pain

作　　者：杨慧[1] 李欣[2] 刘静[3] 黄东[4] 颜辉[5] YANG Hui;LI Xin;LIU Jing;HUANG Dong;YAN Hui(Department of Anesthesiology,the Third Xiangya Hospital of Central South University,Changsha,Hunan 410013,China;Department of Pain,the Second Xiangya Hospital of Central South University,Changsha,Hunan 410012,China;Department of Anesthesiology,Hunan Children′s Hospital,Changsha,Hunan 410007,China;Department of Pain,the Third Xiangya Hospital of Central South University,Changsha,Hunan 410013,China;Department of Neurosurgery,the Third Xiangya Hospital of Central South University,Changsha,Hunan 410013,China)

机构地区：[1]中南大学湘雅三医院麻醉科,湖南长沙410013 [2]中南大学湘雅二医院疼痛科,湖南长沙410012 [3]湖南省儿童医院麻醉科,湖南长沙410007 [4]中南大学湘雅三医院疼痛科,湖南长沙410013 [5]中南大学湘雅三医院神经外科,湖南长沙410013

出　　处：《现代医药卫生》2021年第24期4151-4155,共5页Journal of Modern Medicine & Health

基　　金：国家自然科学基金项目(81172546,81771101,82101317)。

摘　　要：目的制备优化的癌性爆发痛小鼠模型,并评价该模型的可靠性。方法根据吗啡和内皮素-1(ET-1)注射的不同次数和天数制备4组模型小鼠,通过比较行为表现和痛阈变化,选出优化的癌性爆发痛模型;通过观察该组小鼠行为变化和不同时间点痛阈变化对该模型的可靠性进行评价。结果造模后第16~18天,四次组(QID组)、三次组(TID组)和两次组(BID组)先后出现吗啡过量表现和痛阈升高,一次组(QD组)镇痛良好,且第3天痛阈下降;QD组在ET-1注射后10 min痛阈迅速下降,30 min痛阈回升。结论连续3 d每天注射吗啡和ET-1各1次建立的癌性爆发痛优化模型与人类癌性爆发痛表现高度相似。Objective To prepare optimized mouse model of breakthrough cancer pain and evaluate the reliability of the model.Methods Four groups of model mice were prepared according to the different times and days of morphine and endothelin-1(ET-1)injections.The optimized mouse model of breakthrough cancer pain was selected via comparing the changes of behavior and pain threshold.The reliability of the optimized model was evaluated by observing the changes of behavior and pain threshold of the group of mice at different time points.Results From the 16th to 18th days after modeling,morphine overdose and pain threshold increased successively in the QID group,the TID group and the BID group.The QD group had good analgesia and the pain threshold decreased on the third day.In the QD group,the pain threshold decreased rapidly at the 10th minute and increased at the 30th minute after ET-1 injection.Conclusion The optimized mouse model of breakthrough cancer pain established by the method of injecting morphine and ET-1 once a day for three consecutive days is highly similar to human breakthrough cancer pain.

关 键 词：癌性爆发痛 骨转移 内皮素-1 吗啡 小鼠 动物模型 优化 

分 类 号：R739.96[医药卫生—肿瘤]