Toll样受体2、Toll样受体4 mRNA和蛋白在急性出血坏死性胰腺炎肝损伤中的表达及意义  

Expression and significance of Toll-like receptor 2,Toll-like receptor 4 mRNA and protein in hepatic injury induced by AHNP

作　　者：蔡青云 杨留生 张贺川 CAI Qingyun;YANG Liusheng;ZHANG Hechuan(Department of Emergency,Zhumadian Central Hospital,Zhumadian,Henan 463000,China)

机构地区：[1]驻马店市中心医院急诊科,河南驻马店463000

出　　处：《安徽医药》2022年第1期82-86,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨Toll样受体2(TLR2)、TLR4信使核糖核酸(mRNA)和蛋白在急性出血坏死性胰腺炎(AHNP)肝损伤中的表达及意义。方法2019年6月至12月选取健康SD大鼠(郑州大学实验动物中心)60只为研究对象,采用随机数字表法分为模型组和对照组,每组30只,同时模型组和对照组再随机分为3 h组、6 h组和12 h组各10只。模型组采用牛磺胆酸钠建立AHNP模型,对照组注射生理盐水,检测各组血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和淀粉酶,qRT-PCR和Western blotting检测肝脏组织TLR2、TLR4 mRNA和蛋白表达,HE染色观察肝脏组织。结果模型组造模后3 h组、6 h组和12 h组血清ALT[(170.02±32.21)U/L、(310.01±80.02)U/L、(720.11±112.28)U/L]、AST[(450.13±100.21)U/L、(980.12±132.21)U/L、(1100.32±145.03)U/L]和淀粉酶[(7099.27±1209.02)U/L、(11010.19±1200.01)U/L、(17003.21±2007.82)U/L],肝脏组织TLR2和TLR4 mRNA及蛋白相对表达量[TLR2 mRNA(0.962±0.201)、(1.201±0.223)、(1.501±0.212),TLR2蛋白(0.713±0.091)、(1.102±0.100)、(1.343±0.121);TLR4 mRNA(1.701±0.332)、(2.011±0.350)、(2.562±0.313),TLR4蛋白(1.210±0.170)、(1.446±0.182)、(1.788±0.190)]均逐渐增高(P<0.001),且均明显高于对照组[ALT(84.40±14.92)U/L、(85.50±15.20)U/L、(84.19±14.49)U/L;AST(170.02±20.01)U/L、(168.82±21.14)U/L、(171.14±20.11)U/L;淀粉酶(1102.00±183.29)U/L、(1154.49±190.04)U/L、(1160.03±186.27)U/L;TLR2 mRNA(0.030±0.010)、(0.031±0.011)、(0.029±0.009),TLR2蛋白(0.021±0.008)、(0.020±0.009)、(0.022±0.010);TLR4 mRNA(0.882±0.181)、(0.880±0.170)、(0.883±0.179),TLR4蛋白(0.961±0.192)、(0.967±0.181)、(0.966±0.180)](P<0.001)。肝组织TLR2、TLR4 mRNA表达与血清ALT、AST和淀粉酶呈正相关(P<0.05),TLR2、TLR4蛋白表达与血清ALT、AST和淀粉酶呈正相关(P<0.05);模型组造模3 h后可见肝细胞气球样变性,6 h后炎性细胞浸润汇管区,小叶周边细胞发生嗜酸性变性,12 h后出现大片状出血坏死及�Objective To investigate the expression and significance of Toll-like receptor 2(TLR2),Toll-like receptor 4(TLR4)mRNA and protein in liver injury of acute hemorrhagic necrotizing pancreatitis(AHNP).Methods Sixty healthy SD rats in Experimental Animal Center of Zhengzhou University from June to December 2019 were selected as subjects.The rats were assigned into model group and control group by random number table method,with 30 rats in each group.Meanwhile,the model group and control group were randomly divided into 3 h group,6 h group and 12 h group with 10 rats in each group.The model group was established with SODIUM taurocholate to establish AHNP model,while the control group was injected with normal saline.Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST)and amylase of the different groups were detected.The mRNA and protein expressions of TLR2 and TLR4 in liver tissues were detected by QRT-PCR and Western blotting,and the liver tissues were observed by HE staining.Results Serum ALT[(170.02±32.21)U/L,(310.01±80.02)U/L,(720.11±112.28)U/L],AST[(450.13±100.21)U/L,(980.12±132.21)U/L,(1100.32±145.0)U/L]and amylase[(7099.27±1209.02)U/L,(11010.19±1200.01)U/L,(17003.21±2007.82)U/L],TLR2 and TLR4 mRNA and protein relative expression levels in liver tissues[TLR2 mRNA(0.962±0.201),(1.201±0.223),(1.501±0.212),TLR2 protein(0.713±0.091),(1.102±0.100),(1.343±0.121);TLR4 mRNA(1.701±0.332),(2.011±0.350),(2.562±0.313),TLR4 protein(1.210±0.170),(1.446±0.182),(1.788±0.190)]of the model group 3 h,6 h and 12 h after modeling were increased gradually(P<0.001).And they were significantly higher than the control group[ALT(84.40±14.92)U/L,(85.50±15.20)U/L,(84.19±14.49)U/L;AST(170.02±20.01)U/L,(168.82±21.14)U/L,(171.14±20.11)U/L;Amylase(1102.00±183.29)U/L,(1154.49±190.04)U/L,(1160.03±186.27)U/L;TLR2 mRNA(0.030±0.010),(0.031±0.011),(0.029±0.009),TLR2 protein(0.021±0.008),(0.020±0.009),(0.022±0.010);TLR4 mRNA(0.882±0.181),(0.880±0.170),(0.883±0.179),TLR4 protein(0.961±0.192),(0.967

关 键 词：胰腺炎 急性坏死性 肝功能不全 丙氨酸转氨酶 TOLL样受体2 TOLL样受体4 信使核糖核酸 蛋白 大鼠 Sprague-Dawley 

分 类 号：R576[医药卫生—消化系统] R575[医药卫生—内科学]