肥胖小鼠骨髓间充质干细胞的成骨分化  

Osteogenic differentiation of bone marrow mesenchymal stem cells in obese mice

作　　者：陈迟迟 张雨 何家辰[1,2] 施勤 Chen Chichi;Zhang Yu;He Jiachen;Shi Qin(Medical College of Soochow University,Department of Orthopedics,First Affiliated Hospital of Soochow University,Suzhou 215123,Jiangsu Province,China;Institute of Orthopedics,Soochow University,Suzhou 215006,Jiangsu Province,China)

机构地区：[1]苏州大学医学部,苏州大学附属第一医院骨科,江苏省苏州市215123 [2]苏州大学骨科研究所,江苏省苏州市215006

出　　处：《中国组织工程研究》2022年第24期3846-3851,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金面上项目(81972059),项目负责人:施勤。

摘　　要：背景:骨髓间充质干细胞分化受到多种因素的影响,肥胖小鼠骨髓间充质干细胞具有更佳的成骨分化能力。目的:探究正常饮食小鼠与高脂饮食诱导的肥胖小鼠骨髓间充质干细胞成骨分化的差异。方法:将4-6周龄Balb/c小鼠随机分为正常饮食组和高脂饮食组,饲养20周后,通过全骨髓培养法得到骨髓间充质干细胞,成骨诱导7 d后进行碱性磷酸酶染色以及qRT-PCR检测成骨相关基因的表达,成骨诱导14 d后进行茜素红染色。高脂饲养20周后,Micro-CT分析股骨骨量的变化,小鼠股骨切片进行苏木精-伊红染色。结果与结论:①与正常饮食组小鼠相比,高脂饮食饲养20周后小鼠体质量显著增加;②与正常饮食组小鼠骨髓间充质干细胞相比,高脂饮食组小鼠骨髓间充质干细胞中碱性磷酸酶阳性表达和钙结节数目显著增加,成骨相关基因碱性磷酸酶、骨桥蛋白和RUNT相关转录因子2表达水平升高;③Micro-CT重建和骨形态参数量化结果显示,与正常饮食组小鼠相比,高脂饮食组小鼠骨量显著增加,其中骨密度、骨体积分数、骨面积与骨体积比值、骨小梁厚度和骨小梁数目均显著增加,而骨小梁分离度显著减小,苏木精-伊红染色显示高脂饮食组小鼠股骨内含有较多的骨小梁;④结果表明,肥胖小鼠骨髓间充质干细胞成骨分化能力增强,进一步导致小鼠的骨密度、骨小梁厚度和骨小梁数目显著增加,骨量显著升高。BACKGROUND:The differentiation of bone marrow mesenchymal stem cells is affected by many factors.The bone marrow mesenchymal stem cells of obese mice have better osteogenic differentiation ability.OBJECTIVE:To explore the difference of osteogenic differentiation of bone marrow mesenchymal stem cells from obese mice induced by high fat diet and normal diet mice.METHODS:Balb/c mice aged 4-6-weeks were randomly divided into normal diet group and high-fat diet group for 20 weeks.Bone marrow mesenchymal stem cells were isolated by whole bone marrow culture.After 7 days of osteogenic induction,alkaline phosphatase staining and qRT-PCR were performed to detect the expression of osteogenic related genes.At 14 days after osteogenic induction,alizarin red staining was performed.After 20 weeks of high-fat feeding,changes of bone mass of femur were analyzed by Micro-CT.The decalcified sections of the mouse femur were stained with hematoxylin and eosin.RESULTS AND CONCLUSION:(1)Compared with the mice in the normal diet group,after 20 weeks of high-fat feeding,the weight of the mice increased significantly.(2)Compared with the bone marrow mesenchymal stem cells of mice in the normal diet group,the expression of alkaline phosphatase and the number of calcium nodules in the bone marrow mesenchymal stem cells of mice in the high-fat diet group increased significantly,and the expression levels of osteogenic related genes alkaline phosphatase,osteopontin and RUNT-related transcription factor 2 elevated.(3)The results of Micro-CT reconstruction and bone morphological parameter quantification showed that compared with the normal diet group,the high-fat diet group had a significant increase in bone mass,including bone mineral density,bone volume fraction,bone area to bone volume ratio,bone trabecular thickness,and bone trabecular number increased significantly,while the trabecular separation significantly reduced.Hematoxylin-eosin staining showed that there were more trabecular bones in the femur of the high-fat diet group.(4)It is co

关 键 词：干细胞 骨髓间充质干细胞 正常饮食 高脂饮食 肥胖 成骨诱导 成骨分化 小鼠 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]