lncRNA PROX1-AS1负调控miR-206对鼻咽癌细胞SUNE1增殖、迁移侵袭的影响  

Effect of LncRNA PROX1-AS1 on the proliferation,migration and invasion of nasopharyngeal carcinoma cells SUNE1 by negatively regulating miR-206

作　　者：袁小卫 王彦君[2] 熊健 何学祥 YUAN Xiao-wei;WANG Yan-jun;XIONG Jian(Department of Otorhinolaryngology,Wuhan Dongxihu District People’s Hospital,Wuhan 430040,China;Department of Otolaryngology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)

机构地区：[1]武汉市东西湖区人民医院耳鼻喉科,湖北武汉430040 [2]华中科技大学同济医学院附属协和医院耳鼻喉科,湖北武汉430022

出　　处：《中国实验诊断学》2021年第11期1644-1648,共5页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨长链非编码RNA(lncRNA)PROX1反义RNA1(PROX1-AS1)是否靶向miR-206影响鼻咽癌细胞SUNE1的增殖、迁移和侵袭。方法采用实时定量PCR(RT-qPCR)检测鼻咽癌组织和癌旁组织中PROX1-AS1和miR-206表达。双荧光素酶报告实验和RT-qPCR分析PROX1-AS1和miR-206靶向关系。细胞计数试剂盒(CCK-8)法、克隆形成实验、Transwell实验检测PROX1-AS1和miR-206表达对SUNE1细胞增殖活力、克隆形成数、迁移和侵袭细胞数的影响。蛋白质印迹法(Western blot)分析上皮细胞钙粘蛋白(E-cadherin)、和神经钙黏素(N-cadherin)蛋白表达。结果鼻咽癌组织中PROX1-AS1表达较癌旁组织显著升高,miR-206表达较癌旁组织显著降低(P<0.05)。PROX1-AS1直接靶向miR-206并负调控其表达。干扰PROX1-AS1表达后SUNE1细胞活力、克隆形成数、迁移和侵袭细胞数、N-cadherin蛋白表达量显著降低,E-cadherin蛋白表达量显著升高(P<0.05)。与抑制PROX1-AS1比较,同时抑制PROX1-AS1和miR-206后SUNE1细胞活力、克隆形成数、迁移和侵袭细胞数、N-cadherin蛋白表达量显著升高,E-cadherin蛋白表达量显著降低(P<0.05)。结论鼻咽癌中PROX1-AS1呈高表达,干扰PROX1-AS1通过负调控miR-206可抑制鼻咽癌细胞SUNE1的增殖、迁移和侵袭。Objective To investigate whether long-chain non-coding RNA(lncRNA)PROX1 antisense RNA1(PROX1-AS1)targets miR-206 to affect the proliferation,migration and invasion of nasopharyngeal carcinoma cells SUNE1.Methods Real-time quantitative PCR(RT-qPCR)was used to detect the expression of PROX1-AS1 and miR-206 in nasopharyngeal carcinoma tissues and adjacent tissues.The dual luciferase reporter experiment and RT-qPCR were selected to analyse the targeting relationship between PROX1-AS1 and miR-206.The cell counting kit(CCK-8)method,clone formation experiment,and Transwell experiment were used to detect the effects of PROX1-AS1 and miR-206 expression on the proliferation,colony formation,migration and invasion of SUNE1 cells.The expression of epithelial cell cadherin(E-cadherin)and nerve cadherin(N-cadherin)proteins were detected by Western blot.Results The expression of PROX1-AS1 in nasopharyngeal carcinoma tissue was significantly higher than that in adjacent tissues,whereas the expression of miR-206 was significantly lower than that in adjacent tissues(P<0.05).PROX1-AS1 directly targets miR-206 and negatively regulates its expression.After interfering with the expression of PROX1-AS1,the viability of SUNE1 cells,the number of clone formation,the number of migration and invasion cells,the expression of Ncadherin protein were significantly reduced,while the expression of E-cadherin protein was significantly increased(P<0.05).Compared with the inhibition of PROX1-AS1,the viability of SUNE1 cells,the number of colonies,the number of migration and invasion cells,the expression of N-cadherin protein were significantly increased after simultaneously inhibiting PROX1-AS1 and miR-206,whereas the expression of E-cadherin protein was significantly reduced(P<0.05).Conclusion PROX1-AS1 is highly expressed in nasopharyngeal carcinoma.Interfering with PROX1-AS1 can inhibit the proliferation,migration and invasion of nasopharyngeal carcinoma cells SUNE1 by negative regulation of miR-206.

关 键 词：PROX1-AS1 miR-206 鼻咽癌 细胞增殖 迁移 侵袭 

分 类 号：R739.6[医药卫生—肿瘤]