光声显微成像引导注射微整形术研究  

Photoacoustic Microscopy for Injection Navigation of Microplastic Surgery

作　　者：张芬 张吴昱 李春澍 童壮壮 马远征 熊科迪 Zhang Fen;Zhang Wuyu;Li Chunshu;Tong Zhuangzhuang;Ma Yuanzheng;Xiong Kedi(MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science,College of Biophotonics,South China Normal University,Guangzhou,Guangdong 510631,China;Guangdong Provincial Key Laboratory of Laser Life Science,College of Biophotonics,South China Normal University,Guangzhou,Guangdong 510631,China)

机构地区：[1]华南师范大学生物光子学研究院,激光生命科学教育部重点实验室暨激光生命科学研究所,广东广州510631 [2]华南师范大学生物光子学研究院,广东省激光生命科学重点实验室,广东广州510631

出　　处：《中国激光》2021年第21期111-117,共7页Chinese Journal of Lasers

基　　金：国家自然科学基金(62005084,61822505,11774101,61627827);广东省自然科学基金(2019A1515011399);中国博士后科学基金(2019M652943)。

摘　　要：注射微整形手术中可能会出现经皮针头扎破面部动脉血管的情况,这会导致注射药物(如玻尿酸)渗入血管形成栓子、引发血管栓塞,从而致使局部组织缺血、失明甚至中风。为解决这一问题,提出利用光声显微成像引导注射微整形手术。利用带有导针器的光声显微成像探头对固定角度插入的注射针头进行成像,得到针头的行进路线,然后对目标区域进行三维血管成像,通过图像融合并提取其中针头与血管相交区域的像素点数量,可判断针头是否会扎破面部动脉血管,降低手术风险。通过叶脉和活体小鼠成像实验验证了该方法的可行性,结果表明,该方法可以精确地引导扎针,在提高注射微整形手术安全性方面有良好的应用前景。Objective In microplastic surgery,hyaluronic acid is injected into the dermis layer under the wrinkle depression to achieve the wrinkle removal effect.However,the percutaneous needle can puncture facial arteries,causing the penetration of hyaluronic acid into the blood vessels to form emboli and induce vascular embolism,resulting in local tissue ischaemia,blindness,and even stroke.The visualisation of human facial blood vessels can solve this problem.Photoacoustic imaging is a mesoscopic imaging method with high specificity and contrast based on optical absorption differences and ultrasound information carriers.This method combines the advantages of high resolution of optical imaging and large penetration depth of ultrasonic imaging.It has been widely studied in the biomedical imaging field.As an important branch of photoacoustic imaging,photoacoustic microscopy can obtain high-resolution and high-contrast images of blood vessels in the dermis layer of the human skin in a noninvasive and label-free manner.Therefore,a photoacoustic microscopy was proposed to navigate injection-based microplastic surgeries.Methods The needle inserted at a fixed angle was imaged using aphotoacoustic microscopic probe to obtain the injection point of the needle.Then,the three-dimensional(3 D)vascular imaging of the target area was performed.Based on the image fusion and quantitative evaluation,it can be assessed whether the needle will puncture the facial arteries,thereby reducing the risk of surgery.In this study,the feasibility of this method is confirmed using leaf vein imaging and in vivo mouse-ear imaging.Image reconstructions were performed using a user-defined programme,LABVIEW(2019,National Instruments,USA).The three-dimensional images and image fusion were obtained using functions provided by ImageJ(National Institutes of Health,USA).The user-defined algorithm implemented in MATLAB(R2019 b,MathWorks,USA)was used to calculate the number of pixels in the intersection area of theimage fusion.Results and Discussions Leaf veins w

关 键 词：生物光学 光声显微成像 手术导航 注射微整形术 图像融合 

分 类 号：Q631[生物学—生物物理学]