Genome-wide identification and characterization of long non-coding RNAs in Tribolium castaneum  

作　　者：Liu Yang You-Wei Wang Yao-Yao Lu Bin Li Ke-Ping Chen Cheng-Jun Li 

机构地区：[1]School of Life Sciences,Jiangsu University,Zhenjiang,Jiangsu Province 212013,China [2]Jiangsu Key Laboratory for Biodiversity and Biotechnology,College of Life Sciences,Nanjing Normal University,Nanjing,210023,China

出　　处：《Insect Science》2021年第5期1262-1276,共15页昆虫科学（英文版）

基　　金：National Natural Science Foundation of China(Nos.31601893,31572326 and 31572467);Young Talents Training Program of Jiangsu University,and Postgraduate Research&Practice Inno-vation Program of Jiangsu Province(KYCX19_1576).

摘　　要：Long non-coding RNAs(lncRNAs)are poorly understood in insects.In this study,we performed genome-wide analysis of lncRNAs in Tribolium castaneum by RNA-seq.In total,4516 lncRNA transcripts corresponding to 3917 genes were identified from late embryos,early larvae,late larvae,early pupae,late pupae and early adults of T.castaneum,including 3152 novel lncRNAs and 1364 known lncRNAs.These lncRNAs have few exons and transcripts,and are short in length.During development,they exhibited nine different expression patterns.Functionally,they can act either by targeting messenger RNAs(1813 lncRNAs)and lncRNAs(45 lncRNAs)or as micro RNA(miRNA)precursors(46 lncRNAs).LncRNAs were observed to target the metabolic enzymes of glycolysis,TCA cycle and amino acids,demonstrating that lncRNAs control metabolism by regulating metabolic enzymes.Moreover,lncRNAs were shown to participate in cell differentiation and development via their targets.As miRNA precursors,lncRNAs could participate in the ecdysone signaling pathway.This study provides comprehensive information for lncRNAs of T.castaneum,and will promote functional analysis and target identification of lncRNAs in the insect.

关 键 词：GLYCOLYSIS lncRNA METABOLISM TCA cycle Tribolium castaneum 

分 类 号：Q96[生物学—昆虫学]