Development of two continuous hemocyte cell sublines in the Asian corn borer Ostrinia furnacalis and the identification of molecular markers for hemocytes  

作　　者：Jian Hu Yan Du Meng Meng Yipei Dong Jiewen Peng 

机构地区：[1]School of Agriculture,Sun Yat-Sen University,Guangzhou,510275,China [2]State key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-Sen University,Guangzhou,510275,China

出　　处：《Insect Science》2021年第5期1382-1398,共17页昆虫科学（英文版）

基　　金：the National Key R&D Program of China(2017YFD200400);Guangdong Basic and Applied Basic Research Foundation(2020A 1515010455);the National Natural Science Foundation of China(31272091);the Key Project of Chinese National Programs for Fundamental Research and Development(973 programs,2013CB 127603);the International Foundation for Scicncc(C3680-2).

摘　　要：Granulocytes and plasmatocytes play important roles in clearing foreign objects in insects,but it is difficult to distinguish between them in immune reactions.Based on the hemocyte cell line SYSU-OfHe-C established at our lab,two cell sublines,SYSU-OfHe-C Granulocyte(Gr cells)and SYSU-OHe-C Plasmatocyte(PI cells),which possess the morphological characteristics of granulocytes and plasmatocytes,respectively,were established.Gr and PI cells showed different behaviors in immune reactions,such as spreading,phagocytosis and encapsulation.PI cells were easier to spread,but Gr cells tended to undergo aggregation,indicating that they may take different strategies to clear foreign objects.These results also suggested that granulocytes and plasmatocytes may express some different proteins.By comparing the gene expression in cells from the two sublines,1662 differentially expressed genes were identified,and 13 out of 30 transmembrane proteins highly ex pressed in PI cells(six)or Gr cells(seven)were further screened and confirmed by reverse-transcription polymerase chain reaction(PCR).Finally,three transmembrane genes specifically expressed in Pl cells and two transmembrane genes specifically expressed in Gr cells were screened out based on their expressions in immune reactions by quantitative PCR analysis.These genes may potentially be used as molecular markers to distinguish between granulocy tes and plasmatocytes in Ostrinia fiurnacalis,and further to clarify the functions of immune hemocytes in cellular immune reaction such as encapsulation and so on.

关 键 词：cell sublines GRANULOCYTE HEMOCYTE insect molecular marker PLASMATOCYTE 

分 类 号：Q966[生物学—昆虫学]