江汉鸡群体FSHβ基因遗传多态位点分析  

Analysis on genetic polymorphisms of FSHβ gene in Jianghan chicken population

作　　者：黄涛 潘爱銮[1] 申杰[1] 吴艳[1] 蒲跃进[1] 张昊[1] 孙静[1] 梁振华[1] 杜金平[1] 朱昌友[2] 皮劲松[1] HUANG Tao;PAN Ai-luan;SHEN Jie;WU Yan;PU Yue-jin;ZHANG Hao;SUN Jing;LIANG Zhen-hua;DU Jin-ping;ZHU Chang-you;PI Jin-song(Institute of Animal Husbandry and Vetervinary,Hubei Academy of Agricultural Sciences/Hubei Innovation Center of Agricultural Science and Technology/Hubei Key Lab of Animal Embryo Technology and Molecular Breeding,Wuhan 430064,China;Hubei Animal Husbandry Breeding Farm,Wuhan 434010,China)

机构地区：[1]湖北省农业科学院畜牧兽医研究所/湖北省农业科技创新中心/动物胚胎与分子育种湖北省重点实验室,武汉430064 [2]湖北省畜牧良种场,武汉434010

出　　处：《湖北农业科学》2021年第23期127-130,共4页Hubei Agricultural Sciences

基　　金：湖北省畜禽优质增效与绿色养殖技术创新行动(2018skjcx05);湖北省农业科学院青年基金项目(2020NKYJJ11)。

摘　　要：利用基因组重测序技术对江汉鸡群体卵泡雌激素(FSH)β亚基基因序列进行全面筛选,结果表明,该群体内一共发现35个SNPs和2个indels,其中cds区1个SNP突变造成甘氨酸变成丝氨酸。结合生物信息学软件分析基因保守序列,预测M6A修饰位点、调控元件以及miRNA靶向序列,结果发现7个SNPs位于潜在的调控元件序列中,7个SNPs和1个indel位于潜在miRNA结合序列中。所筛选的上述遗传变异位点可能参与了FSHβ基因的表达调控,在江汉鸡繁殖特性中扮演一定的角色。In this study,the genome-wide re-sequencing technology was applied in Jianghan chicken population for genetic variation identification,the results showed that,35 single nucleotide polymorphisms(SNPs)and 2 indels in theβsubunit gene of follicular estrogen(FSH)were found,one single-base mutation in the cds could cause glycine to serine.Combining with bioinformatics softwares,M6A modification site prediction,regulatory element prediction and miRNA targeting sequence prediction were further analyzed.It was found that 7 single-base mutations were located in the potential regulatory element sequence,and 7 single-base mutations and 1 indel were located in the potential miRNA binding sequence.Those genetic mutations may be involved in the regulation of FSHβgene expression,and play a certain role in the genetic characteristics of Jianghan chicken.

关 键 词：FSHΒ SNP 江汉鸡 

分 类 号：S831.2[农业科学—畜牧学] Q754[农业科学—畜牧兽医]