基于团体标准T/CALAS 21-2017的Wistar大鼠微卫星DNA群体遗传质量分析  

Population Genetic Quality Analysis of Microsatellite DNA in Wistar Rats Based on T/CALAS 21-2017

作　　者：魏杰[1] 左琴[1] 王洪[1] 李欢 周佳琪 光姣娜 范涛[1] 刘佐民[1] 付瑞[1] 岳秉飞[1] WEI Jie;ZUO Qin;WANG Hong;LI Huan;ZHOU Jiaqi;GUANG Jiaona;FAN Tao;LIU Zuomin;FU Rui;YUE Bingfei(National Institutes for Food and Drug Control,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院,北京102629

出　　处：《实验动物与比较医学》2021年第6期528-534,共7页Laboratory Animal and Comparative Medicine

摘　　要：目的利用团体标准T/CALAS 21—2017推荐的方法对不同时期封闭群Wistar大鼠的遗传质量进行分析,同时进行团体标准T/CALAS 21—2017的适用性评价。方法2015年、2019年选测的Wistar大鼠分别命名为A组和B组。按团体标准T/CALAS 21—2017要求,使用25对微卫星引物获取两组封闭群Wistar大鼠的遗传参数进行质量分析,利用多态性信息含量(polymorphism information content,PIC)分析位点多态性。结果A组和B组大鼠中分别检测到100个和69个等位基因。两群体平均杂合度分别为0.574和0.447,平均PIC分别为0.541和0.393。结论团体标准T/CALAS 21—2017在封闭群大鼠遗传质量分析中具有良好的适用性,A组Wistar大鼠的遗传多样性优于B组。Objective To analyze the genetic quality of an outbred stock of Wistar rats in different periods by T/CALAS 21-2017 method,and to evaluate the applicability of the association standard.Methods Wistar rats selected from the same outbred stock in 2015 and 2019 were named as group A and group B,respectively.Twenty-five pairs of microsatellite primers were used to get genetic parameters for quality analysis of the outbred stock Wistar rats according to the association standard T/CALAS 21-2017.Polymorphism information content(PIC)was used to analyze the polymorphism of the loci.Results One hundred alleles in group A and 69 alleles in group B were obtained.The average heterozygosity of the two groups was 0.574 and 0.447,while the average PIC was 0.541 and 0.393,respectively.Conclusion The association standard T/CALAS 21-2017 has good applicability in the genetic quality analysis of outbred stock rats,and the genetic diversity of group A is better than that of group B.

关 键 词：WISTAR大鼠 T/CALAS 21-2017 封闭群 微卫星DNA 遗传多样性 

分 类 号：Q95-33[生物学—动物学] R-332[医药卫生]