近地层臭氧浓度升高对亲子代大豆叶片抗氧化系统的影响  

Effect of Ozone Concentration Increasing Near the Ground on Antioxidant System of Parent and Offspring Soybean Leaves

作　　者：周映彤 王岩[1] 孙铭禹 伞昱 姚星州 赵天宏[1] ZHOU Yingtong;WANG Yan;SUN Mingyu;SAN Yu;YAO Xingzhou;ZHAO Tianhong(College of Agronomy,Shenyang Agricultural University,Shenyang 110000,China)

机构地区：[1]沈阳农业大学农学院,辽宁沈阳110000

出　　处：《生态环境学报》2021年第11期2195-2203,共9页Ecology and Environmental Sciences

基　　金：国家自然科学基金项目(31570404)。

摘　　要：为探究臭氧胁迫对不同世代大豆(Glycine max)抗氧化能力的影响,以栽培大豆“铁丰29号”为试验材料,在开顶式气室(OTCs)中,采用盆栽试验方法,设置3个O_(3)熏蒸体积分数梯度[CK:对照,45nL·L^(−1)O_(3);T1:(80±10)nL·L^(−1)O_(3);T2:(120±10)nL·L^(−1)O_(3)],并将受胁迫后的子代复种,研究臭氧浓度升高条件下大豆亲代和子代抗氧化能力的差异。结果表明,(1)随着臭氧体积分数增加,开花期和结荚期亲子代大豆叶片相对电导率较CK显著增加(P<0.05);MDA含量均增加,且在分枝期和开花期的T2处理下,与CK之间达显著差异(P<0.05),其增加幅度分别为32.9%、37.0%。(2)随着臭氧体积分数增加,O_(2)∙^(−)产生速率加快,除分枝期T1条件的其他处理外,S1和S2较CK都表现出显著差异水平(P<0.05);H_(2)O_(2)含量增大,在分枝期和开花期的T2条件下,S1、S2较CK上升幅度显著,分别达到88.2%、92.4%(P<0.05)。(3)SOD、CAT活性逐渐减弱,POD活性逐步增强。与对照相比,亲子代在3个时期均有显著下降的趋势(P<0.05)。(4)在胁迫后期,子代大豆叶片的ROS含量、产生速率高于亲代,抗氧化酶活性低于亲代,且在T2处理下达到显著水平(P<0.05)。(5)亲、子代大豆的膜脂过氧化程度、活性氧含量和产生速率两两之间均呈极显著正相关(P<0.01),与SOD、CAT活性均极显著负相关(P<0.01),与POD活性呈不相关关系(P>0.05)。试验表明,臭氧体积分数升高对亲子代大豆的抗氧化能力有明显的抑制作用,且对子代的抑制作用略强于亲代,说明同时受到了遗传因素的影响。In order to explore the effect of ozone stress on the antioxidant ability of different generations of Glycine max,three O_(3)fumigation concentration gradients[CK:control,45nL·L^(−1)O_(3);T1:(80±10)nL·L^(−1)O_(3);T2:(120±10)nL·L^(−1)O_(3)]were used to treat soybean in open top chamber(OTCs),and then copied the species after the ozone stress and studied the difference ability of antioxi-dant.The results showed that(1)the relative conductivity in soybean leaves increased significantly between flowering and pod pe-riods compared with CK treatment(P<0.05).MDA content increased and had a significantly difference during the branching,flower-ing stage and T2 condition(P<0.05)by 32.9%and 37.0%with CK treatment,respectively.(2)The O_(2)∙^(−)production rate increased,and both S1 and S2 showed significantly different level except for branch T1 conditions(P<0.05);the H_(2)O_(2)content increased,and the S1 and S2 increased significantly at T2 during the branching and flowering stages,reaching 88.2%and 92.4%respectively.(3)SOD,CAT activity gradually decreased and POD activity gradually increased,which showed a significantly decline in all three stages compared with the CK treatment(P<0.05).(4)In the later stage,the ROS content and generation rate in offspring soybean leaves were higher than the parental;the oxidase activity in offspring soybean leaves was lower than the parental,and reached significantly levels under T2 condition(P<0.05).(5)The degree of membrane lipid peroxidation,active oxygen content and production rate were significantly positively correlated between parent and offspring soybeans(P<0.01),significantly negatively associated with SOD,CAT activity(P<0.01)and unrelated to POD activity(P>0.05).The results show that increased ozone concentration significantly inhibit the antioxidant capacity of soybeans,and the offspring slightly stronger than the parent,which indicate that it is also affected by genetic factors.

关 键 词：臭氧浓度升高 活性氧 抗氧化 大豆 亲子代 

分 类 号：X171.5[环境科学与工程—环境科学]