基于明胶膜基底捕获和原位培养循环肿瘤细胞  

Capture and culture in situ of circulating tumor cells based on gelatin film substrate

作　　者：张珂[1] 张陶冶 黄慧明[1] ZHANG Ke;ZHANG Taoye;HUANG Huiming(School of Physics and Technology,Wuhan University,Wuhan 430072,China)

机构地区：[1]武汉大学物理科学与技术学院,湖北武汉430072

出　　处：《中国医学物理学杂志》2021年第12期1549-1553,共5页Chinese Journal of Medical Physics

基　　金：武汉应用基金会前沿项目(2019010701011386);深圳市知识创新计划基础研究项目(JCYJ20180302173424902)。

摘　　要：目前从血液中分离得到高活力的循环肿瘤细胞(CTCs)仍面临一定的挑战,本研究设计了一个明胶膜基底,可以同时实现捕获和原位培养CTCs。该明胶基底对CTCs的捕获效率最高可达86.8%。由于明胶具有良好的生物相容性,明胶膜基底在捕获到CTCs后可直接进行原位培养,减少了释放过程中对细胞的损害,有利于后续细胞分析。该明胶膜基底有望在临床CTCs检测中发挥作用。At present,there are some challenges in obtaining circulating tumor cells(CTCs)with high viability from blood.Herein a gelatin film substrate is designed for realizing the capture and culture in situ of CTCs.The maximum capture efficiency of the proposed substrate for CTCs reaches 86.8%.Because of the good biocompatibility of gelatin,CTCs can be directly cultured in situ after being captured by gelatin film substrate,which reduces the damages to the cells during the release process and is more beneficial for subsequent cellular analysis.The gelatin film substrate is expected to play a role in the clinical detection of CTCs.

关 键 词：循环肿瘤细胞 明胶膜基底 原位培养 

分 类 号：R-33[医药卫生]