一种基于近红外的半夏炮制品快速鉴别方法  

Fast Identification of Processed Pinellia Ternate by Near Infrared Spectroscopy

作　　者：黄露 周勤梅[1] 熊亮[1] 彭成[1] 耿昭 郭力[1] 曹小玉[1] Huang Lu;Zhou Qinmei;Xiong Liang;Peng Cheng;Geng Zhao;Guo Li;Cao Xiaoyu(Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China;Sichuan Provincial Institute for Food and Drug Control,Chengdu,611137,China)

机构地区：[1]成都中医药大学,四川成都611137 [2]四川省食品药品检验检测院,四川成都611137

出　　处：《成都中医药大学学报》2021年第4期50-53,61,共5页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省科技计划重点研发项目(2018JZ0081);四川省中医药管理局科技专项资助项目(2018C019);四川省青年科技创新研究团队专项计划项目(2017TD0001)。

摘　　要：目的:分析法半夏、姜半夏、清半夏的近红外光谱,建立半夏不同炮制品的快速鉴别法。方法:采集半夏炮制品近红外光谱信息,结合二阶导数光谱、化学计量学技术,探究不同半夏炮制品的光谱差异及建立快速鉴别模型。结果:在近红外光谱中,半夏炮制品在8336.91、6861.57、6311.60、5861.42、5668.34、5162.24、4772.29、4330.16 cm^(-1)处均有较强的振动吸收,表明半夏炮制品的主要化学组分较为相似。二阶导数光谱结合聚类、偏最小二乘法判别分析和K最近邻分类算法分析发现,半夏炮制品之间具有特征性差异。讨论:近红外光谱能快速有效地用于鉴别半夏炮制品,为半夏炮制工艺、质量评价提供科学的参考依据。Objective:To establish a rapid identification for processed Pinellia ternate by analying the near-infrared spectrum of Fa Banxia,Jiang Banxia and Qing Banxia.Methods:The study collected spectrum of processed Pinellia ternata,combined with second-order reciprocal spectrum and chemometrics techniques to explore the spectrum differences of processed Pinellia ternata and establish a rapid identification model.Results:In the near-infrared spectroscopy,processed Pinellia ternata had strong vibration absorption at 8333.91,6861.57,6131.60,5861.42,5686.34,5162.24,4772.29,4330.16 cm^(-1),indicating that the main chemical components of processed Pinellia ternata were relatively similar.The results of second-order derivative spectroscopy analysis of processed Pinellia ternata,clustering,PLS-DA,and KNN showed that there were characteristic differences between processed Pinellia ternata.Discussion:Near-infrared spectroscopy can quickly and effectively be used to identify processed Pinellia ternate,which provides a scientific reference for the processing technology and quality evaluation of Pinellia ternate.

关 键 词：半夏炮制品 近红外光谱 聚类分析 PLS-DA KNN 鉴别 

分 类 号：R283[医药卫生—中药学] O433[医药卫生—中医学]