耐碱性硫酸盐还原菌发酵培养基的优化研究  

Optimization of fermentation medium for alkali-resistant sulfates-reducing bacteria

作　　者：姚亮 赵运生 孔京 孔凡敏 胡永红[1] YAO Liang;ZHAO Yun-sheng;KONG Jing;KONG Fan-min;HU Yong-hong(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211816,China;Sinopec Nanjing Research Institute of Chemical Industry Co.,Ltd.,Nanjing 210048,China)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211816 [2]中石化南京化工研究院有限公司,江苏南京210048

出　　处：《现代化工》2021年第12期154-159,共6页Modern Chemical Industry

基　　金：微生物法脱除工业气体中二氧化硫及硫磺回收(202010112)。

摘　　要：以耐碱性硫酸盐还原菌硫降解率为指标,比较基础碱性培养基不同的碳源、(有机与无机)氮源、无机盐的降解效果,筛选其中效果较好的8个因素,优化1株耐碱性硫酸盐还原菌的发酵工艺;设计PB试验并分析其中影响最为显著的3个因素;进行最陡爬坡实验逼近因素的最佳质量浓度,以最陡爬坡实验降解率最高的组为中心值进行中心组合试验,并分析得到最佳优化因素为:乙醇质量浓度为5.27 g/L、酵母粉质量浓度为2.03 g/L、KCl质量浓度为4.02 g/L。在此条件下,降解率达到81.21%,较未优化前提高17.99%。Taking the degradation rate of alkali-resistant sulfates-reducing bacteria to sulfur as the index, the effects of different carbon sources, organic and inorganic nitrogen sources, and inorganic salts in basic alkaline medium are compared, 8 factors with better effects are screened, and the fermentation process of one alkali-resistant sulfates-reducing bacteria is optimized.PB tests are designed to analyze three factors with the most significant effect.The steepest climbing experiment is carried out to approach the best mass concentration of the factor.Taking the group with the highest degradation rate in the steepest climbing test as the central value, central combination test is carried out, and the optimization factors are obtained through analysis.The best factors are as follows: the mass concentrations of ethanol, yeast powder and KCl are 5.27 g·L^(-1),2.03 g·L^(-1)and 4.02 g·L^(-1),respectively.Under the best conditions, the degradation rate reaches 81.21%,which is 17.99% higher than that before optimization.

关 键 词：生物脱硫 硫酸盐还原菌 响应面 二氧化硫降解 发酵优化 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]