抗癌痛方对肺癌骨痛小鼠的作用机制及对症因子的影响  

Study on the Mechanism of Anti-cancer Recipe in Mice with Lung Cancer and Bone Pain and Its Influence on Inflammatory Factors

作　　者：陈训春 李名兰 潘碧云 王燕英 CHEN Xun-chun;LI Ming-lan;PAN Bi-yun;WANG Yan-ying(Haikou People′s Hospital,Haikou Hainan 570208)

机构地区：[1]海口市人民医院,海南海口570208

出　　处：《世界中西医结合杂志》2021年第10期1826-1830,共5页World Journal of Integrated Traditional and Western Medicine

基　　金：海南省卫生厅基金资助项目(琼卫2017LX-23)。

摘　　要：目的研究抗癌痛方对通过胫骨内注射Walker 256细胞建立的癌症诱导性骨痛(CIBP)大鼠模型的抗伤害作用。方法将96只4周龄SPF级雌性SD大鼠随机分成A组(16只)、B组(16只)、C组(64只)。A组大鼠不做任何处理,B组大鼠左后腿胫骨注射无菌生理盐水15~20μl,C组大鼠左后腿胫骨注射肺癌细胞Walker 256建立CIBP模型。C组按照灌胃药物分为C1、C2、C3、C4组,每组16只。造模后14 d、15 d、16 d连续对C1组大鼠灌胃生理盐水(2 g/ml),C2组、C3组、C4组大鼠分别灌胃0.3 g/ml、0.6 g/ml、1.2 g/ml抗癌痛方。观察每次灌胃前、灌胃后30 min、灌胃后1 h大鼠的机械痛阈值。取B组大鼠10只,随机分为B1组和B2组两组,每组各5只,B1组不做任何处理,B2组给予抗癌痛方1.2 g/ml灌胃,连续3 d。取C1组大鼠10只,随机分为C1-1组和C1-2组两组,每组各5只,C1-1组不做任何处理,C1-2组给予抗癌痛方1.2 g/ml灌胃,连续3 d。采用Western Blot方法测量大鼠脊髓IL-1β、IL-6和TNF-α的蛋白质表达情况。结果A组和B组大鼠各时点的机械痛阈值比较,差异均无统计学意义(P>0.05)。C组大鼠术后7 d、10 d和14 d机械痛阈值较A组和B组大鼠同期明显降低,差异有统计学意义(P<0.05),且C组大鼠术后7 d、10 d和14 d机械痛阈值比较,差异无统计学意义(P>0.05)。C组大鼠术后7 d、10 d和14 d机械痛阈值较术前1 d和术后1 d、3 d和5 d明显降低,差异有统计学意义(P<0.05)。与灌胃前比较,C2组、C3组和C4组大鼠术后14 d、15 d、16 d灌胃后30 min和灌胃后1 h的机械痛阈明显升高,差异有统计学意义(P<0.05)。C2组、C3组和C4组大鼠术后14 d、15 d、16 d灌胃后30 min和灌胃后1 h的机械痛阈较C1组均明显升高,差异有统计学意义(P<0.05)。C3组和C4组大鼠术后14 d、15 d、16 d灌胃后30 min和灌胃后1 h的机械痛阈较C2组明显升高,差异有统计学意义(P<0.05)。与B1组比较,B2组的脊髓IL-1β、IL-6和TNF-α蛋白质表达明显降低,Objective The aim of this study was to evaluate the anti-nociceptive effect of Anti-cancer pain recipe in a rat model of cancer-induced bone pain(CIBP)established by intra-tibia injection of Walker 256 cells.Methods Ninety-six SPF female SD rats were randomly divided into group A(16),group B(16),and group C(64).Rats in group A did not do any treatment,rats in group B were injected with sterile saline 15-20μL into the tibia of the left hind leg,rats in group C were injected with lung cancer cells Walker 256 into the tibia of the left hind leg to establish a CIBP model.Group C was divided into groups C1,C2,C3,and C4 according to intragastric drugs,with 16 rats in each group.After modeling,14 days,15 days,and 16 days,rats in group C1 were given normal saline(2 g/ml).Rats in group C2,C3,and C4 were given 0.3 g/ml,0.6 g/ml,and 1.2 g/ml anticancer pain recipe,respectively.Observe the mechanical pain threshold of rats before,30 min after gavage,and 1 h after gavage.Ten rats in group B were randomly divided into two groups,group B1 and group B2,with 5 rats in each group.Group B1 did not do any treatment,group B2 was given Anti-cancer pain recipe 1.2 g/ml gavage for 3 consecutive days.Take 10 rats in group C1 and randomly divide them into two groups:C1-1 group and C1-2 group,with 5 rats in each group.Group C1-1 does not do any treatment.Group C1-2 is given Anti-cancer pain recipe.2 g/ml intragastrically for 3 consecutive days.Western Blot method was used to measure the protein expression of IL-1β,IL-6 and TNF-αin rat spinal cord.Results There was no statistically significant difference in the mechanical pain threshold of rats in group A and group B at each time point(P>0.05).The mechanical pain threshold of rats in group C was significantly lower than that of rats in group A and B at the same time at 7 d,10 d,and 14 d after operation(P<0.05),and rats in group C were mechanically painful at 7 d,10 d and 14 d after operation.There was no statistically significant difference in pain threshold(P>0.05).The mechanical pain t

关 键 词：癌症诱导性骨痛 抗癌痛方 促炎症性细胞因子 

分 类 号：R734.2[医药卫生—肿瘤]