褪黑素对人神经胶质瘤U118细胞的抗增殖作用及机制研究  

Anti-proliferation effect of melatonin on human glioma U118 cells and its mechanism

作　　者：李鑫[1] 吕子阳 刘佳佳[1] 王珊[1] LI Xin;LYU Zi-yang;LIU Jia-jia;WANG Shan(Department of Pharmaceutical Engineering,School of Chemistry and Chemical Engineering,Central South University,Changsha 410083)

机构地区：[1]中南大学化学化工学院制药工程系,长沙410083

出　　处：《中南药学》2021年第12期2483-2488,共6页Central South Pharmacy

基　　金：国家自然科学基金项目(No.81872919)。

摘　　要：目的研究褪黑素(MT)对人神经胶质瘤U118细胞增殖能力及膜联蛋白A2(ANXA2)和促癌蛋白c-Myc表达的影响。方法用不同浓度的MT(1 nmol·L^(-1),100 nmol·L^(-1),10 μmol·L^(-1),1 mmol·L^(-1))处理U118细胞后,CCK-8法检测各组细胞活性。用1 mmol·L^(-1)MT处理U118细胞后,EDU染色法检测各组细胞DNA合成情况,克隆形成法检测各组细胞克隆数,Western blot检测增殖标记物Ki67和PCNA的表达情况及ANXA2和c-Myc的蛋白表达情况,RT-qPCR检测ANXA2的mRNA的表达情况。结果 MT可以抑制U118细胞增殖,并且可以明显降低U118细胞中ANXA2和c-Myc的表达水平。结论 MT可能通过降低ANXA2及下游通路c-Myc的表达从而抑制U118细胞的增殖。Objective To determine the effect of melatonin (MT) on the proliferation of human glioma U118 cells and the expression of Annexin A2 (ANXA2) and oncoprotein c-Myc.Methods After the U118 cells were treated with different concentrations of MT (1 nmol·L^(-1),100 nmol·L^(-1),10 μmol·L^(-1),and 1 mmol·L^(-1)),the cell viability of each group was detected by CCK-8.The DNA synthesis of the cells in each group was detected by EDU staining,and the colony number of cells in each group was detected by colony formation,the expressions of proliferation markers Ki67 and PCNA,and the protein expressions of ANXA2 and c-Myc were detected by Western blot,and the mRNA expression of ANXA2 was detected by RT-qPCR,after treated with 1 mmol·L^(-1)MT.Results MT inhibited the proliferation of U118 cells and significantly decreased the expression level of ANXA2 and c-Myc in U118 cells.Conclusion MT may inhibit the proliferation of U118 cells by decreasing the expression of ANXA2 and the downstream pathway c-Myc.

关 键 词：褪黑素 胶质瘤 增殖 膜联蛋白A2 

分 类 号：R739.41[医药卫生—肿瘤] R975[医药卫生—临床医学]