猝灭-恢复型电化学发光传感器测定抗坏血酸的研究  

Determination of ascorbic acid by electrochemiluminescence sensor based on quenching-recovery method

作　　者：王琪 李超[1] 张召香[1] WANG Qi;LI Chao;ZHANG Zhaoxiang(Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science,MOE,College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042)

机构地区：[1]光电传感与生命分析教育部重点实验室青岛科技大学化学与分子工程学院,青岛266042

出　　处：《分析试验室》2021年第12期1365-1369,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21105051)资助。

摘　　要：制备了氮硫掺杂石墨烯量子点(NSGQDs),并将其修饰在金电极表面。基于NSGQDs良好的电化学发光(ECL)特性,以其为探针,进一步将铜-金属有机框架(Cu-MOF)修饰于该电极表面,构建了猝灭-恢复型ECL传感器用于抗坏血酸的高灵敏检测。由于NSGQDs表面存在大量氨基,当Cu-MOF存在时,NSGQDs的ECL信号猝灭;加入抗坏血酸后,Cu-MOF从NSGQDs表面自动脱落,NSGQDs的ECL信号得以恢复。NSGQDs的ECL信号恢复值与抗坏血酸浓度在0.1~40μmol/L之间呈良好的线性关系,检出限为3.2 nmol/L。该法已用于血样中抗坏血酸的测定,并进一步研究了人血液中抗坏血酸24 h的药代动力学规律。Nitrogen and sulfur co-doped graphene quantum dots(NSGQDs) were prepared and adsorbed on the surface of the Au working electrode. Based on its excellent electrochemiluminescence(ECL) properties, a quenching-recovery ECL method was constructed by modifying this electrode with Cu-MOF using the NSGQDs as probe. Under the action of external voltage, the NSGQDs transit from ground state to excited state, and release light energy in the process of returning to the ground state. Due to the presence of a large number of amino groups on the surface of NSGQDs, the ECL of NSGQDs was quenched when Cu-metal organic framework(MOF) is present. The presence of ascorbic acid(AA) resulted in the departure of Cu-MOF from the surface of the NSGQDs, and the ECL signal of NSGQDs recovered. Under the optimal conditions, the recovered ECL value of NSGQDs was linearly related with the concentration of ascorbic acid in the range of 0.1-40 μmol/L, and the detection limit was 3.2 nmol/L. It has been applied to the determination of ascorbic acid in practical samples with satisfactory results.The pharmacokinetics of ascorbic acid in human blood for 24 h was further studied.

关 键 词：氮硫掺杂石墨烯量子点 铜-金属有机框架 电化学发光 抗坏血酸 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]