生命素一号抗人胚肺MRC-5成纤维细胞衰老的机制  

Mechanism of anti-cellular senescence of Shengming-1 on human embryonic lung MRC-5 fibroblast cells in vitro

作　　者：王金彩 陈阳[1] 何琪杨[1] Wang Jincai;Chen Yang;He Qiyang(Institute of Medicinal Biotechnology,Beijing Union Medical College,Chinese Academy of Medical Sciences,Beijing,100050,P.R.China)

机构地区：[1]中国医学科学院北京协和医学院医药生物技术研究所,北京100050

出　　处：《老年医学与保健》2021年第6期1130-1133,1143,共5页Geriatrics & Health Care

基　　金：国家自然科学基金(31471150)。

摘　　要：目的研究营养素混合物生命素一号是否具有抗细胞衰老作用。方法以过氧化氢诱导的人胚肺MRC-5细胞为衰老模型,使用标准抗氧化剂N-乙酰半胱氨酸(NAC)作为阳性对照,CCK-8法检测细胞增殖,衰老特异的β-半乳糖苷酶染色确定衰老细胞,流式细胞仪检测活性氧自由基,蛋白印迹方法检测衰老相关的蛋白表达。结果与阳性药物NAC作用类似,生命素一号单独作用细胞,也具有一定的促进细胞增殖作用(P<0.01);较强的拮抗过氧化氢引起的细胞增殖抑制作用(P<0.01)。生命素一号明显地减少过氧化氢诱导的衰老细胞(P<0.01),降低活性氧自由基水平,抑制衰老相关的p53、p16和p27的表达。结论生命素一号具有抗细胞衰老作用,其机制与抗氧化及降低衰老相关的蛋白表达相关。Objective To study whether Shengming-1(SM-1),a mixture of various nutrients,can inhibit cellular senescence in vitro.Methods Human embryonic lung MRC-5 fibroblast cells induced by hydrogen peroxide were used as the model of cellular senescence.The standard antioxidant N-acetylcysteine(NAC)was used as a positive control.Cell proliferation was determined by CCK-8 assay,cellular senescence was identified by senescence-specificβ-galactosidase staining,reactive oxygen free radicals were detected by flow cytometry and senescence-related protein expression was detected by Western blot method.Results Similar to the effect of positive drug NAC,SM-1 alone could also promote cell proliferation and antagonize the inhibitory effect of hydrogen peroxide on cell proliferation(P<0.01).SM-1 could significantly reduced the cellular senescence induced by hydrogen peroxide(P<0.01),decreased the level of reactive oxygen free radicals,and inhibit the expression of senescencerelated p53,p16 and p27.Conclusion SM-1 has the efficacy against cellular senescence,and its mechanism is related to antioxidation and reducing the expression of senescence-related proteins.

关 键 词：细胞衰老 生命素一号 抗氧化 N-乙酰半胱氨酸 营养素 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]