ppgA基因在冠突曲霉有性产孢中的功能分析  

Functional Analysis of ppgA Gene in the Sexual Sporulation of Aspergillus cristatum

作　　者：桂小霞 谭玉梅 王亚萍 邵蕾 刘永翔[2] 任锡毅 黄永会[2] 任秀秀 刘作易[2] Gui Xiaoxia;Tan Yumei;Wang Yaping;Shao Lei;Liu Yongxiang;Ren Xiyi;Huang Yonghui;Ren Xiuxiu;Liu Zuoyi(Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB),College of Life Sciences,Institute of Agro-bioengineering,Guizhou University,Guiyang,550025;Guizhou Key Laboratory for Agricultural Biotechnology,Guizhou Institute of Biotechnology,Guizhou Academy of Agricultural Sciences,Guiyang,550006;Agricultural College,Guizhou University,Guiyang,550025;School of Basic Medicine,Guizhou University of Traditional Chinese,Guiyang,550025;Guizhou University of Engineering Science,Guiyang,550025)

机构地区：[1]贵州大学生命科学学院,农业生物工程研究院,山地生态与农业生物工程协同创新中心,山地植物资源保护与保护种质创新教育部重点实验室,贵阳550025 [2]贵州省农业科学院,贵州省生物技术研究所,贵州省农业生物技术重点实验室,贵阳550006 [3]贵州大学农学院,贵阳550025 [4]贵州中医药大学基础医学院,贵阳550025 [5]贵州工程应用技术学院,贵阳550025

出　　处：《分子植物育种》2021年第22期7479-7487,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31960018);黔农科院基础性公益性专项([2021]02);贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]389)共同资助。

摘　　要：为研究信息素前体基因ppgA在冠突曲霉(Aspergillus cristatus)有性产孢中的功能,本研究利用同源重组原理对冠突曲霉高效基因敲除菌株ΔKu70中ppgA基因进行敲除。形态学观察发现,在MYA培养基中,ppgA基因敲除株前期生长较ΔKu70菌株快。培养6 d后,敲除株形成较厚的菌落,浅黄色。此外,敲除株菌落中产生褐色色素的时间较晚,且明显减少。在1 mol/L NaCl条件下,敲除株仅菌落中央形成稀疏成熟闭囊壳,闭囊壳数量减少约15倍,子囊孢子成熟期延长;敲除株菌落边缘闭囊壳不成熟,无子囊孢子产生。在3 mol/L NaCl条件下,敲除株菌落白色,生长缓慢。这些结果表明,ppgA基因在冠突曲霉的有性产孢过程中发挥重要作用,该研究为进一步探讨冠突曲霉有性产孢机制的研究提供了理论参考和数据支持。In order to study the function of pheromone precursor gene ppgA of Aspergillus cristatus on sexual sporulation,ppgA gene was deleted inΔKu70 by homologous recombination.Morphological observation showed that the ppgA knockout strain grew faster than theΔKu70 strain in the early stage on MYA medium.After 6 days of cultivation,the ppgA knockout strain formed thick,pale yellow colonies.Moreover,the ppgA knockout strain delayed and reduced the brown pigment formation.Under 1 mol/L NaCl conditions,the ppgA knockout strain only produced few mature cleistothecia in the center of the colony.The number of the cleistothecia were reduced about15 times compared to theΔKu70 strain and the maturation period of ascospores were prolonged.The cleistothecia produced on edge of the colony were imature without ascospores.Under 3 mol/L NaCl conditions,the knockout strains formed white colonies and grew slowly.These results indicated that the ppgA gene played an important role in regulating the sexual sporulation of Aspergillus cristatus.This study provides theoretical reference and data support for further research on the mechanism of sexual reproduction of Aspergillus cristatum.

关 键 词：冠突曲霉 ppgA基因 闭囊壳 

分 类 号：Q933[生物学—微生物学]