发用化妆品微生物检验方法适用性研究  

Applicability of Microbiological Testing Methods for Hair Cosmetics

作　　者：刘奕 罗俊 庄意琼 王晓炜 应国红 LIU Yi;LUO Jun;ZHUANG Yiqiong;WANG Xiaowei;YING Guohong(Shenzhen Institute for Drug Control/NMPA Key Laboratory for Monitoring and Evaluation of Cosmetics,Shenzhen 518057,Guangdong,China)

机构地区：[1]深圳市药品检验研究院/国家药品监督管理局化妆品监测评价重点实验室,广东深圳518057

出　　处：《香料香精化妆品》2021年第6期5-9,共5页Flavour Fragrance Cosmetics

摘　　要：通过对发用化妆品微生物检验方法学研究,探讨化妆品微生物检验方法适用性试验的必要性。按现行《中国药典》1105和1106的要求,对7批发用化妆品进行微生物检验方法适用性研究。结果发现:按常规法进行方法适用性试验,结果仅3批能通过,其余4批需采用培养基稀释法或薄膜过滤法,试验菌的回收率才能达到0.5~2.0。致病菌检验中,有3批需采用培养基稀释法(20、50 mL/tube)进行耐热大肠菌群检查,1批需采用培养基稀释法(500 mL/bottle)进行金黄色葡萄球菌检查。这7批发用化妆品中5个品种有不同程度的抑菌现象,影响微生物检验结果的准确性,因此化妆品微生物检验前进行方法适用性试验很有必要。The necessity of applicability test of microbiological testing methods for cosmetics was discussed by studying its methodology for hair cosmetics.Following the criterion of Chinese Pharmacopoeia 1105 and 1106,the applicability of microbiological testing methods for 7 hair cosmetics was studied.It was found that according to the conventional method,3 samples met the requirements of applicability in the microbial counting test.In order to ensure the recovery rate of test bacteria between 0.5 and 2.0,the culture medium dilution method or membrane filtration method should be applied to eliminate the bacteriostatic activity for other 4 samples.For the pathogenic bacteria,heat-resistant coliform of 3 samples was examined by medium dilution method(20,50 mL/tube),Staphylococcus aureus of one sample was detected by medium dilution method(500 mL/bottle).In 7 batches of hair cosmetics,5 batches presented bacteriostatic activity,which affected the accuracy of microbiological test results.Therefore,it is necessary to verify the applicability of methods before the microbiological test for cosmetics.

关 键 词：发用化妆品 微生物检验方法适用性 抑菌性 

分 类 号：TQ658.3[化学工程—精细化工]