CircRNA hsa_circ_0044569在肺癌诊断中的价值  

CircRNA hsa_circ_0044569 as A Potential Biomarker for Diagnosis of Lung Cancer

作　　者：侯岩君 田进海[1,3] 王立斌[1,3] 何进喜[1] HOU Yanjun;TIAN Jinhai;WANG Libin;HE Jinxi(Department of General Thoracic Surgery,The General Hospital of Ningxia Medical University,Yinchuan 750004,China;Department of Cardiovascular Surgery,Second Affiliated Hospital of Hainan Medical College,Haikou 570311,China;Department of Beijing National Biochip Research Center Sub-center in Ningxia,The General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学总医院普胸外科,银川750004 [2]海南医学院第二附属医院心血管外科,海口570311 [3]宁夏医科大学总医院生物芯片国家工程中心宁夏分中心,银川750004

出　　处：《肿瘤防治研究》2021年第12期1061-1065,共5页Cancer Research on Prevention and Treatment

基　　金：宁夏留学人员创新创业项目(2018-12);国家自然科学基金(81860470);宁夏重点研发计划对外科技合作重点项目(2019BFH02012);宁夏高层次科技创新领军人才项目(KJT2019003);宁夏生物芯片技术研发创新团队(2019-18);宁夏医科大学科学研究基金资助项目(XM2018099)。

摘　　要：目的使用生物芯片技术筛选出在肺癌中具备诊断价值的标志物。方法通过circRNA表达谱芯片筛选4对在肺癌中差异表达的circRNA,在另外52对肺癌患者的组织标本中,采用qRT-PCR方法验证筛选的差异circRNA hsa_circ_0044569,同时收集患者临床病例信息。用SPSS23.0对所得数据进行独立样本t检验和ROC曲线分析,获得肺癌患者临床资料与circRNA hsa_circ_0044569表达的关系。结果hsa_circ_0044569在肺癌组织中的表达水平高于与其配对的癌旁组织。ROC曲线分析结果显示,hsa_circ_0044569诊断肺癌的临界值值为9.62,AUC为0.758,P<0.05,敏感度0.712,特异性0.712。hsa_circ_0044569表达与肺癌的病理类型之间具有相关性(P<0.05)。结论hsa_circ_0044569在肺癌中高表达,并与肺癌的病理类型具有相关性,提示其在肺癌早期诊断中具备成为潜在生物标志物的可能。Objective To screen out the biomarkers with diagnostic value in lung cancer by biochip technology.Methods We screened four pairs of differentially-expressed circRNAs in lung cancer by circRNA expression profiling chip,and then verified the screened differential circRNA hsa_circ_0044569 by qRT-PCR,and collected clinical case information of patients at the same time.The independent sample t test and ROC curve were used to analyze the relation between the clinical data of lung cancer patients and the expression of circRNA hsa_circ_0044569 on clinical samples.Results The expression level of hsa_circ_0044569 in lung cancer tissues was higher than that of its paired adjacent tissues.The cutoff value of hsa_circ_0044569 for the diagnosis of lung cancer was 9.62,AUC was 0.758,P<0.05,sensitivity was 0.712,and specificity was 0.712.The expression of hsa_circ_0044569 was significantly related with the pathological type of lung cancer patients(P<0.05).Conclusion hsa_circ_0044569 is highly expressed in lung cancer and related to the pathological type,suggesting that it may become a potential biomarker in the early diagnosis of lung cancer.

关 键 词：circRNA 生物标志物 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]