低温对水稻突变体cisc(ta)苗期抗氧化系统和氨基酸积累的影响  

Effects of Low Temperature Stress on Antioxidant System and Amino Acid Accumulation of Rice Mutant cisc(ta)at Seedling Stage

作　　者：龚晓平 况晓明 罗挺 李加胜 莫春红 何焕然 赵许兵 余伟 张致力 GONG Xiao-ping;KUANG Xiao-ming;LUO Ting(Yudongnan Academy of Agricultural Sciences,Chongqing 408000)

机构地区：[1]重庆市渝东南农业科学院,重庆408000

出　　处：《安徽农业科学》2021年第24期55-59,共5页Journal of Anhui Agricultural Sciences

基　　金：重庆市基础研究与前沿探索项目(cstc2018jcyjAX0447)。

摘　　要：[目的]对低温胁迫下苗期水稻(Oryza sativa L.)进行耐冷性生理学和形态学鉴定,为耐冷相关基因定位和克隆奠定基础。[方法]以耐冷籼稻材料P1211和冷敏型籼稻突变体cisc(ta)为试验材料,于苗期在人工气候箱中进行低温胁迫处理(12℃),以适温处理(25℃)的参试材料作为对照,研究低温胁迫对水稻冷敏感突变体cisc(ta)苗期抗氧化系统和氨基酸积累的影响。[结果]与适温处理相比,P1211和cisc(ta)在低温下幼苗株高、冠鲜重、冠干重、最长根长、根鲜重、根干重显著下降;同时叶片抗氧化酶活性(SOD、POD、CAT),可溶性蛋白,光合色素含量不同程度降低,冷敏型籼稻突变体cisc(ta)幼苗的降幅大于耐冷籼稻材料P1211;此外,在长期低温胁迫下,突变体cisc(ta)叶片丙二醛含量极显著增加,而P1211无显著差异。低温胁迫后P1211和突变体cisc(ta)的脯氨酸、精氨酸、天冬氨酸、谷氨酸、赖氨酸含量极显著增加,而亮氨酸、甲硫氨酸、异亮氨酸的含量极显著降低,其中突变体cisc(ta)精氨酸的含量增幅最大。恢复正常处理后,突变体cisc(ta)叶片光合色素含量恢复到正常(CK)水平,但株高显著变矮,成熟期结实率和产量降低。[结论]低温胁迫处理影响突变体cisc(ta)幼苗生长特性,降低了叶片抗氧化酶活性,提高了脯氨酸和游离氨基酸的含量。该研究加深了对水稻苗期耐冷性生理基础的全面认识,并为相关基因的克隆及水稻耐冷分子育种奠定基础。[Objective]To identify the physiological and morphological of cold tolerance of rice(Oryza sative L.)at seedling stage under low temperature stress,so as to lay a foundation for the localization and cloning of cold tolerance related genes.[Method]Using cold-tolerant indica rice material P1211 and cold-sensitive indica rice mutant cisc(ta)as test materials,low-temperature stress treatment(12℃)was carried out in an artificial climate incubator at the seedling stage,and suitable temperature treatment(25℃)was used as the test material.The test materials were used as controls to study the effects of low temperature stress on the antioxidant system and amino acid accumulation in the seedling stage of the cold-sensitive mutant cisc(ta)of rice.[Result]Compared with the suitable temperature treatment,the seedling height,shoot fresh weight,shoot dry weight,longest root length,root fresh weight and root dry weight of P1211 and cisc(ta)were significantly reduced under low temperature;at the same time,the activities of antioxidant enzymes(SOD,POD,CAT),soluble protein and photosynthetic pigment in leaves decreased in varying degrees.The decline of cold sensitive indica rice mutant cisc(ta)seedlings was greater than that of cold tolerant indica rice material P1211;in addition,under long-term low temperature stress,the content of malondialdehyde in leaves of mutant cisc(ta)increased significantly,but there was no significant difference in P1211.After low temperature stress,the contents of proline,arginine,aspartate,glutamate and lysine in P1211 and mutant cisc(ta)increased significantly,while the contents of leucine,methionine and isoleucine decreased significantly.The content of arginine experienced the biggest growth in the cisc(ta)mutant.After the normal treatment was restored,the photosynthetic pigment content of the mutant cisc(ta)leaves returned to the normal(CK)level,but the plant height was significantly shorter,and the seed setting rate and yield at the maturity stage were reduced.[Conclusion]Low temperature stress

关 键 词：水稻 低温胁迫 耐冷性 抗氧化系统 游离氨基酸 

分 类 号：S511[农业科学—作物学]