慢加速与快加速旋转手法对香草素受体4/一氧化氮通路抑制强度的比较  

Observation on the Efficacy of TRPV4/NO Expression in DRG by Slowly-Accelerating or Fastly-Accelerating Types of Spinal Manipulation on Acute Radiculopathy in Rat Model

作　　者：韩雪[1] 张军[1] 韩磊[1] 耿进朝[1] 崔莹[1] HAN Xue;ZHANG Jun;HAN Lei;GENG Jinchao;CUI Ying(Wangjing Hospital of Chinese Academy of Traditional Chinese Medical Sciences,Beijing 100102,China)

机构地区：[1]中国中医科学院望京医院,北京100102

出　　处：《中国中医骨伤科杂志》2021年第12期22-25,31,共5页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：国家自然科学基金面上项目(81373657);国家自然科学基金青年项目(81503602)。

摘　　要：目的:比较慢加速与快加速旋转手法对大鼠腰椎背根节(DRG)损伤模型机械痛敏、TRPV4通道蛋白和亚硝酸盐的影响,探讨不同加速度载荷旋转手法的消炎祛痛效果。方法:192只雄性SD大鼠随机分为低速-零加速旋转手法组、低速-慢加速旋转手法组、低速-快加速旋转手法组、阳性药物灌胃对照组,每组各48只。所有大鼠均经L_(5~6)椎间孔外口先插入钢棒"致压"、后植入髓核"致炎"的方法建立DRG损伤疼痛模型,分别施行模拟L_(5~6)关节假手法、模拟L_(5~6)关节松动手法、模拟L_(5~6)关节调整手法、COX-2抑制药Etoricoxib内服。每组各取6只大鼠于术前1 d及术后第1,7,15,21天行机械性撤足痛阈值(PWT)的测定;每组各取24只大鼠于术后第9天和第21天时取出手术同侧的L_(5~6)DRG,其中18只用于TRPV4通道蛋白表达的检测;6只用于NO代谢产物亚硝酸盐含量的检测。结果:低速-快加速组50%PWT在术后第7天时开始高于低速-零加速组,差异有统计学意义(P<0.05);第15天和第21天时更高,差异有统计学意义(P<0.01)。低速-慢加速组50%PWT在术后第1天时高于低速-快加速组,差异有统计学意义(P<0.05);但第7,15,21天时两者并无差别,差异无统计学意义(P>0.05)。低速-慢加速组与Etoricoxib组相比较并无差别,差异无统计学意义(P>0.05)。经腰椎旋转手法干预后,SNK检验显示在术后第9天和第21天时低速-慢加速组、低速-快加速组、Etoricoxib组的TRPV4蛋白平均灰度值、亚硝酸盐含量两两比较并无差异,但均明显低于低速-零加速组(P<0.01)。结论:慢加速与快加速模拟手法对TRPV4/NO通路的抑制强度无差异,均与口服Etoricoxib的效果相当。在其他力学影响因素不变的情况下,不同的加速度载荷并不影响最终的消炎祛痛效果。Objective:To compare the efficacies of fastly-accelerating and slowly-accelerating types of spinal manipulation on mechanical hyperalgesia, TRPV4 channel protein expression and nitrite content in rat dorsal root ganglion(DRG) injury model with acute radiculopathy and to explore the anti-inflammatory and analgesic efficacies of rotating manipulation under different acceleration loads.Methods:192 male SD rats were randomly divided into low-speed and no-accelerating manipulation group, low-speed and fastly-accelerating manipulation group, low-speed and slowly-accelerating manipulation group and positive medicine gavage control group, with 48 rats in each group.In all rats, steel rods were inserted into the outer orifice of L_(5~6)intervertebral foramen to induce pressure, then nucleus pulposus were applied to induce inflammation, and finally DRG injury models were established.Four groups were performed Simulated L_(5~6)joint sham manipulation, simulated L_(5~6)joint loosening manipulation, simulated L_(5~6)joint adjustment manipulation and oral administration of Etoricoxib, a COX-2 inhibitor.Six rats in each group were selected to measure the 50% mechanical paw withdrawal threshold(PWT)one day before modeling and 1,7,15,21 d after modeling.The left L_(5~6)DRG of 24 rats in each group were collected on days 9,21 respectively after surgery to detect the expression of TRPV4 with 18 rats and nitrite content with 6 rats in each group.Results:The 50%PWT in the low-speed and fastly-accelerating group was higher than that in the low-speed and no-accelerating manipulation group on the 7 th day after operation(P<0.05) and was higher at the 15 th and 21 st day(P<0.01).The 50% PWT in the low-speed and slowly-accelerating manipulation group was higher than that in the low-speed and fastly-accelerating group on the 1 st day after operation(P<0.05),but there was no difference between the two groups on the 7 th, 15 th and 21 st days(P>0.05).There was no difference between the low-speed and slowly-accelerating manipulation group and

关 键 词：旋转手法 加速度 香草素受体4/一氧化氮 疼痛 

分 类 号：R-33[医药卫生]