基于生物信息学分析HLA-DMA基因在LGG中的表达和功能  

Expression and Function of HLA-DMA Gene in LGG Based on Bioinformatics Analysis

作　　者：李泽艳 王晓旋 来明名 马雪艳 LI Ze-yan;WANG Xiao-xuan;LAI Ming-ming;MA Xue-yan(School of Basic Medical Sciences,Dali University,Dali 671000,Yunnan,China)

机构地区：[1]大理大学基础医学院,云南大理671000

出　　处：《医学信息》2021年第24期21-26,共6页Journal of Medical Information

基　　金：国家自然科学基金地区基金项目(编号:82160504)。

摘　　要：目的基于生物信息学方法探讨HLA-DMA基因在低级别脑胶质瘤(LGG)中的表达和功能。方法基于GEPIA数据库分析HLA-DMA在LGG和正常组织中的表达差异;从TCGA数据库中下载正常组织和LGG组织基因表达数据和临床资料,采用R软件分析HLA-DMA表达水平与临床病理特征和预后的关系;利用Coexpedia构建HLA-DMA在LGG中的共表达基因网络,并用FunRich软件对共表达基因进行注释;借助GSEA探讨HLA-DMA参与的信号通路。结果LGG组织中HLA-DMA表达高于正常组织,差异有统计学意义(P<0.05),且与肿瘤分级和预后相关;单、多因素Cox分析显示,HLA-DMA可作为LGG患者的独立预后因素(P<0.05);HLA-DMA与共表达基因所参与的分子生物学功能主要与免疫调节相关;GSEA结果显示,HLA-DMA高表达主要富集于免疫调节相关通路和凋亡通路。结论HLA-DMA在LGG中呈高表达,可作为LGG治疗和预后评估的潜在靶点。Objective To explore the expression and function of HLA-DMA gene in low-grade glioma(LGG)based on bioinformatics methods.Methods The expression differences of HLA-DMA in LGG and normal tissues were analyzed based on GEPIA database.The gene expression data and clinical data of normal tissues and LGG tissues were downloaded from TCGA database.The relationship between HLA-DMA expression level and clinicopathological features and prognosis was analyzed by R software.The co-expression gene network of HLA-DMA in LGG was constructed by Coexpedia and annotated by FunRich software.The signaling pathway involved in HLA-DMA were explored by GSEA.Results The expression of HLA-DMA in LGG tissues was higher than that in normal tissues,and the difference was statistically significant(P<0.05),which was related to tumor grade and prognosis.Univariate and multivariate Cox analysis showed that HLA-DMA was an independent prognostic factor for LGG patients(P<0.05).The molecular biological functions of HLA-DMA and co-expression genes are mainly related to immune regulation;gSEA results showed that the high expression of HLA-DMA was mainly enriched in immune regulation-related pathways and apoptosis pathways.Conclusion HLA-DMA is highly expressed in LGG,which can be used as a potential target for LGG treatment and prognosis evaluation.

关 键 词：LGG HLA-DMA 免疫调节 

分 类 号：R739.41[医药卫生—肿瘤]