基于柠檬酸-铕纳米配位聚合物构建荧光探针快速检测汤煲中5’-肌苷酸含量  

Rapid Detection of Inosine-5’-monophosphate in Soup Based on Citrate/Eeuropium Nano-coordination Polymer as a Fluorescence Probe

作　　者：高田毅 孙冲[2] 朱宏星 黄杨 曹锦轩[1] 王道营[2] GAO Tianyi;SUN Chong;ZHU Hongxing;HUANG Yang;CAO Jinxuan;WANG Daoying(Key Laboratory of Food Deep Processing Technology of Animal Protein of Zhejiang Province,College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo 315800,China;Institute of Agri-products Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]宁波大学食品与药学学院,浙江省动物蛋白食品精深加工技术重点实验室,浙江宁波315800 [2]江苏省农业科学院农产品加工研究所,江苏南京210014

出　　处：《食品科学》2021年第24期283-288,共6页Food Science

基　　金：国家自然科学基金面上项目(31972137);省部共建国家重点实验室培育基地-江苏省食品质量安全重点实验室项目(2019JG001)。

摘　　要：基于柠檬酸-铕金属有机纳米配体聚合物(citrate/europium lanthanide coordination polymer nanoparticles,Cit/Eu LCP NPs)构建快速检测肉品汤煲中5’-肌苷酸(inosine-5’-monophosphate,5’-IMP)的荧光探针。研究结果表明,5’-IMP对Cit/Eu LCP NPs有良好的荧光猝灭作用。在最佳条件下,该荧光探针在5’-IMP 2.5~200μg/mL的质量浓度范围内呈现出良好的线性关系,检出限为0.17μg/mL,且具备良好的抗干扰、稳定性和重复性。为了验证方法可行性,将该方法应用于实际鸡汤样品中的5’-IMP检测,测得加标回收率为97.85%~103.95%,可为快速检测肉品汤煲中5’-IMP提供新的思路和方法。In this study,a fluorescent probe based on citrate/europium lanthanide coordination polymer nanoparticles(Cit/Eu LCP NPs)was constructed to detect inosine-5’-monophosphate(5’-IMP)in soup.The results showed that 5’-IMP could quench the fluorescence of Cit/Eu LCP NPs.Under the optimal conditions,the fluorescence probe showed a good linear relationship with 5’-IMP in the concentration range of 2.5–200μg/mL with a detection limit of 0.17μg/mL.Meanwhile,the fluorescent probe had good anti-interference ability,stability and repeatability.In order to verify its feasibility,Cit/Eu LCP NPs was applied to the determination of 5’-IMP in a real chicken soup sample,indicating that the recoveries of the added 5’-IMP was 97.85%–103.95%.Therefore,the Cit/Eu LCP NPs-based fluorescent probe can be used for the rapid detection of 5’-IMP in soup.

关 键 词：镧系金属 配位聚合物 荧光探针 检测分析 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]