Biological activities of the Ornithogalum orthophyllum and its in silico ADMET profile  

作　　者：Duygu Taşkın ŞeydaŞentürk Eray Metin Güler Ahmet Doğan Gülden Zehra Omurtag Mizgin Ermanoğlu Turgut Taşkın 

机构地区：[1]Department of Analytical Chemistry,Hamidiye Faculty of Pharmacy,University of Health Sciences Turkey,Istanbul 34668,Turkey [2]Darıca Leyla Pharmacy,Kocaeli 41700,Turkey [3]Hamidiye School of Medicine,Department of Medical Biochemistry,University of Health Sciences Turkey,Istanbul 34668,Turkey [4]Department of Pharmaceutical Botany,Faculty of Pharmacy,Marmara University,Istanbul 34854,Turkey [5]Department of Pharmaceutical Toxicology,School of Pharmacy,Istanbul Medipol University,Istanbul 34810,Turkey [6]Department of Pharmacognosy,Faculty of Pharmacy,Marmara University,Istanbul 34854,Turkey

出　　处：《Traditional Medicine Research》2021年第6期59-66,共8页TMR传统医学研究

摘　　要：Background:The bulbs and aerial parts of Ornithogalum are used in Turkey both as food and to treat various ailments,and some of its medical applications are well known.However,the biological activities of Ornithogalum orthophyllum have not yet been investigated.The objective of this study was to examine the antioxidant,urease and cholinesterase enzyme inhibition,and cytotoxic activities of different extracts obtained from the bulb and aerial parts of the O.orthophyllum plant.In addition,the absorption,distribution,metabolism,excretion,and toxicity properties of some phenolic compounds in plants were examined in silico.Methods:The antioxidant activity of the extracts obtained from the aerial parts of O.orthophyllum was investigated using the 2,2-diphenyl-1-picrylhydrazyl,2,2-azinobis(3-ethylbenzothiazoline-6-sulphonic acid),ferric reducing antioxidant power,and cupric reducing antioxidant capacity methods.Urease and cholinesterase enzyme inhibition were determined by using the indophenol and Ellman methods,respectively.The cytotoxic activity of the extracts was measured using a test based on the luminometric readings of the adenosine triphosphate levels of the cells.pkCSM,a free online web server(http://structure.bioc.cam.ac.uk/pkcsm)was used to predict the properties of the compounds analyzed.Results:The methanol extract of the aerial parts of O.orthophyllum was observed to have strong antioxidant and acetylcholinesterase enzyme inhibition potential.The petroleum ether extract of the aerial parts showed the highest anti-urease activity.The chloroform extract of the aerial parts exhibited the highest cytotoxic effect against A431 human epidermoid carcinoma cells.The absorption percentages of protocatechuic acid,p-hydroxybenzoic acid,vanillic acid,and p-coumaric acid compounds from the small intestine were between 71.17%and 93.49%,which were quite high.All the compounds were predicted to be unable to penetrate the central nervous system due to their inability to cross the blood-brain barrier.Not all compounds analy

关 键 词：Ornithogalum orthophyllum ANTIOXIDANT CYTOTOXIC ANTICHOLINESTERASE anti-urease ADMET 

分 类 号：R28[医药卫生—中药学]