产前补充牛磺酸对宫内生长受限幼鼠感觉运动及海马突触素的影响  

Effect of prenatal taurine supplementation on sensorimotor ability and hippocampal synaptophysin expression of juvenile rats with intrauterine growth restriction

作　　者：方琼[1] 刘敬[2] 陈琅[1] 陈巧彬[1] 柯俊[3] 章九云 刘颖[2] 付薇[2] Fang Qiong;Liu Jing;Chen Lang;Chen Qiaobin;Ke Jun;Zhang Jiuyun;Liu Ying;Fu Wei(Department of Pediatrics,Fujian Provincial Hospital,Shengli Clinical Medical College of Fujian Medical University,Fuzhou 350001,China;Department of Neonatology,Beijing Chaoyang Maternal and Child Health Hospital,Beijing 100101,China;Department of Emergency,Fujian Provincial Hospital,Shengli Clinical Medical College of Fujian Medical University,Fujian Provincial Institute of Emergency Medicine,Fuzhou 350001,China)

机构地区：[1]福建医科大学省立临床医学院,福建省立医院儿科,福州350001 [2]北京市朝阳区妇幼保健院新生儿科,北京100101 [3]福建医科大学省立临床医学院,福建省立急诊内科,福建省急诊医学研究所,福州350001

出　　处：《中华实用儿科临床杂志》2021年第23期1806-1810,共5页Chinese Journal of Applied Clinical Pediatrics

基　　金：福建省卫生健康科技计划项目(2019-ZQN-11,2019-CXB-16)。

摘　　要：目的探讨产前补充牛磺酸对宫内生长受限(IUGR)幼鼠感觉运动能力及海马区突触素表达水平的影响。方法采用妊娠期全程饥饿法建立IUGR鼠模型。孕鼠随机分为正常对照组、IUGR组、IUGR+牛磺酸组。2周龄幼鼠行悬吊实验和平衡木实验,测试其感觉运动能力,2周测试结束后采用免疫组织化学法和Western blot法检测海马区突触素表达,并将幼鼠感觉运动能力与海马区突触素表达量行相关性分析。结果28 d IUGR组幼鼠悬吊实验时间、平衡木实验分数均较正常对照组下降[(14.62±3.46)s比(25.38±5.92)s,P<0.001;(9.08±1.38)分比(12.08±1.16)分,P<0.001];IUGR+牛磺酸组悬吊实验时间[(22.77±5.16)s]、平衡木实验分数[(11.08±1.38)分]均高于IUGR组(均P<0.05),但与正常对照组比较差异无统计学意义(P>0.05)。IUGR组海马区突触素平均吸光度值、蛋白表达均小于正常对照组[(53.96±2.37)%比(61.68±3.07)%,P<0.001;1.82±0.23比2.23±0.17,P<0.001];IUGR+牛磺酸组的平均吸光值[(60.27±2.59)%]、蛋白表达(2.07±0.17)均较IUGR组升高(均P<0.05),但与正常对照组比较差异无统计学意义(P>0.05)。海马区突触素表达与悬吊实验、平衡木实验均呈正相关(均P<0.05)。结论产前补充牛磺酸可通过促进IUGR幼鼠海马区突触素表达,提高幼鼠感觉运动能力。Objective To investigate the effect of prenatal taurine supplementation on sensorimotor ability and synaptophysin(Syn)expression in the hippocampus of juvenile rats with intrauterine growth restriction(IUGR).Methods The IUGR rat model was induced by food restriction throughout pregnancy.Pregnant rats were randomly divided into normal control group,IUGR group and IUGR+taurine group.Sensorimotor ability was tested in 2-week-old juvenile rats via grading the tail suspension scores and beam balance test scores,followed by detecting Syn expression in the hippocampus of juvenile rats by immunohistochemistry and Western blot.The correlation between sensorimotor ability scores and Syn expression was assessed.Results Tail suspension time[(14.62±3.46)s vs.(25.38±5.92)s,P<0.001]and beam balance test scores[(9.08±1.38)scores vs.(12.08±1.16)scores,P<0.001]in the IUGR group were significant lower than those of normal control group.Tail suspension time(22.77±5.16)s and beam balance test scores(11.08±1.38)scores in IUGR+taurine group were significantly higher than those in IUGR group(all P<0.05),but there was no significant difference comparable to those in normal control group(P>0.05).The average optical density(A)value[(53.96±2.37)%vs.(61.68±3.07)%,P<0.001]and protein expression of Syn(1.82±0.23 vs.2.23±0.17,P<0.001)in rat hippocampus of IUGR group were all significantly lower than those in normal control group.The A value[(60.27±2.59)%]and expression of Syn protein(2.07±0.17)in IUGR+taurine group were significantly higher than those in IUGR group(all P<0.05),but there was no significant difference comparable to those in normal control group(P>0.05).The expression of Syn in rat hippocampus was positively correlated with the tail suspension test time and beam balance test scores(all P<0.05).Conclusions Prenatal taurine supplementation can improve the sensorimotor ability of juvenile rats with IUGR by upregulating Syn in the hippocampus.

关 键 词：宫内生长受限 牛磺酸 突触素 海马 感觉运动 

分 类 号：R714.5[医药卫生—妇产科学]