泡沫分离法纯化葫芦巴中薯蓣皂苷及抗氧化性的研究  

Foam Fractionation Optimization and Antioxidant Activity Studies of Dioscin from Trigonella foenum-graecum

作　　者：王志娟 张炜 甘文梅 乜世成 高红 宋林 Wang Zhijuan;Zhang Wei;Gan Wenmei;Nie Shicheng;Gao Hong;Song Lin(School of Chemistry and Chemical Engineering, Qinghai Normal University, Xining 810008)

机构地区：[1]青海师范大学化学化工学院,西宁810008

出　　处：《中国粮油学报》2021年第11期144-150,161,共8页Journal of the Chinese Cereals and Oils Association

基　　金：高原人工湿地生态平衡构建与应用示范(2021-SF-13)。

摘　　要：在超高压法提取葫芦巴中薯蓣皂苷的基础上,建立利用泡沫分离法纯化葫芦巴中薯蓣皂苷的新方法。通过单因素实验及响应面设计实验,研究上样浓度、温度、装液量、气速对泡沫法分离葫芦巴中薯蓣皂苷回收率及富集比的影响,同时研究其体外抗氧化活性。结果得出,泡沫分离薯蓣皂苷的最佳条件为上样质量浓度0.03 mg/mL,温度28℃,气速450 mL/min,装液量400 mL,在此条件下,薯蓣皂苷的回收率为86.57%,富集比为3.82。薯蓣皂苷对DPPH·、·OH、超氧阴离子有较好的清除效果,具有较强的还原能力。研究表明,泡沫分离法是一种有效、简便、易于工业化生产和对环境友好的分离富集葫芦巴中薯蓣皂苷的方法。A new method for purification of dioscin from trigonella foenum-graecum by foam fractionation was established based on the extraction of dioscin by ultrahigh pressure extraction technology.Based on the single factors experiments,the effects of solvent concentration,temperature,volume of liquid and air flow rate on the enrichment ratio and recovery of the dioscin from trigonella foenum-graecum were optimized by response surface methodology(RSM).At the same time,its antioxidant activity in vitro was also studied.The results indicated that 0.03 mg/mL,26℃,400 mL and 450 mL/min were the optimum separation parameters for solvent concentration,temperature,volume of liquid and air flow rate,respectively.Under this condition,the enrichment ratio and recovery of the dioscin were 88.23%and 1.25.The antioxidant activity study showed that abilities of dioscin in trigonella foenum-graecum to scavenge DPPH,hydroxyl radical and superoxide anion were good and also has a strong reduction ability total reducing power assays.The results showed that the foam fractionation method was an effective,simple,easy-to-industrialize and environment-friendly method for the separation and enrichment of dioscin from trigonella foenum-graecum.

关 键 词：泡沫分离法 薯蓣皂苷 葫芦巴 响应面法 体外抗氧化活性 

分 类 号：O629.2[理学—有机化学]