Electrochemical micro-aptasensors for exosome detection based on hybridization chain reaction amplification  

作　　者：Wenfen Zhang Zhenhua Tian Shujie Yang Joseph Rich Shuaiguo Zhao Mikael Klingeborn Po-Hsun Huang Zhishang Li Alexander Stout Quinn Murphy Edward Patz Shusheng Zhang Guozhen Liu Tony Jun Huang 

机构地区：[1]College of Chemistry,Zhengzhou University,Zhengzhou,Henan 450001,People's Republic of China [2]Department of Mechanical Engineering and Materials Science,Duke University,Durham,NC 27708,USA [3]Department of Biomedical Engineering,Duke University,Durham,NC 27708,USA [4]Department of Ophthalmology,Duke University,Durham,NC 27708,USA [5]College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou,Zhejiang 310058,Peopled Republic of China [6]Department of Radiology,Duke University,Durham,NC 27708,USA [7]School of Life and Health Sciences,The Chinese University of Hong Kong,Shenzhen,Guangdong 518172,China

出　　处：《Microsystems & Nanoengineering》2021年第4期161-168,共8页微系统与纳米工程（英文）

基　　金：support of National Institutes of Health(NIH)grants U18TR003778 and R01GM135486;Wenfen Zhang acknowledges the support of the National Natural Science Foundation of China(22004109);the China Scholarship Council.Guozhen Liu acknowledges the support of the Chinese University of Hong Kong,Shenzhen providing the University Development fund and Presidential Fellow fund.Joseph Rich acknowledges that this material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No.1644868.

摘　　要：Exosomes are cell-derived nanovesicles that have recently gained popularity as potential biomarkers in liquid biopsies due to the large amounts of molecular cargo they carry,such as nucleic acids and proteins.However,most existing exosome-based analytical sensing methods struggle to achieve high sensitivity and high selectivity simultaneously.In this work,we present an electrochemical micro-aptasensor for the highly sensitive detection of exosomes by integrating a micropatterned electrochemical aptasensor and a hybridization chain reaction(HCR)signal amplification method.Specifically,exosomes are enriched on CD63 aptamer-functionalized electrodes and then recognized by HCR products with avidin-horseradish peroxidase(HRP)attached using EpCAM aptamers as bridges.Subsequently,the current signal that is generated through the enzyme reaction between the HRP enzyme and 3,3,,5/5,-tetramethylbenzidine(TMB)/H_(2)0_(2) directly correlates to the amount of bound HRP on the HCR products and thus to the number of target exosomes.By introducing anti-EpCAM aptamers,micro-aptasensors can detect cancerous exosomes with high specificity.Due to the micropatterned electrodes and HCR dual-amplification strategy,the micro-aptasensors achieve a linear detection response for a wide range of exosome concentrations from 2.5×10^(3) to 1×10^(7) exosomes/mL,with a detection limit of 5×10^(2) exosomes/mL.Moreover,our method successfully detects lung cancer exosomes in serum samples of early-stage and late-stage lung cancer patients,showcasing the great potential for early cancer diagnosis.

关 键 词：EXOSOME SPECIFICITY ATTACHED 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]