UPLC-MS/MS法同时测定毕氏海蓬子中13个功能成分  

Simultaneous Determination of 13 Functional Constituents in Salicornia bigelovii by UPLC-MS/MS

作　　者：邵阳阳 赵善仓 冯温泽 张梅 郑颜 董燕婕 范丽霞 王磊 李大鹏[3] 冯立田 SHAO Yang-yang;ZHAO Shan-cang;FENG Wen-ze;ZHANG Mei;ZHENG Yan;DONG Yan-jie;FAN Li-xia;WANG Lei;Li Da-peng;FENG Li-tian(Jinan Winsir Biotechnology Co.,Ltd,Jinan 250100,China;Institute of Quality Standard and Testing Technology for Agro-Products,Shandong Academy of Agricultural Sciences,Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety,Jinan 250100,China;College of Food Science and Engineering,Shandong Agricultural University,Tai’an 271018,China;University of Chinese Academy of Sciences,Beijing 100049,China;Institute of Oceanology Chinese Academy of Sciences,Qingdao 266071,China;School of Agricultural Engineering and Food Science,Shandong University of Technology,Zibo 255000,China)

机构地区：[1]济南温泽生物科技有限公司,济南250100 [2]山东省农业科学院农业质量标准与检测技术研究所/山东省食品质量与安全检测技术重点实验室,济南250100 [3]山东农业大学食品科学与工程学院,山东泰安271018 [4]中国科学院大学,北京100049 [5]中国科学院海洋研究所,山东青岛266071 [6]山东理工大学农业工程与食品科学学院,山东淄博255000

出　　处：《中国食物与营养》2021年第12期18-21,71,共5页Food and Nutrition in China

基　　金：山东省农业重大应用技术创新项目(项目编号:SD2019ZZ023)。

摘　　要：利用超高效液相色谱-串联质谱(UPLC-MS/MS)法同时测定毕氏海蓬子(Salicornia bigelovii,SB)中没食子儿茶素、表没食子儿茶素、隐绿原酸、绿原酸、新绿原酸、儿茶素、表儿茶素、异绿原酸-B、芦丁、异槲皮苷、褪黑素、山柰酚-3-O-芸香糖苷、槲皮苷13个功能成分的含量。样品利用甲醇提取,过0.22μm滤膜后上机检测。采用C_(18)色谱柱(100 mm×2.1 mm,1.7μm)进行分离,以0.1%甲酸水溶液-甲醇为流动相,流速0.3 mL/min,柱温(35±5)℃,采用电喷雾离子源(ESI)、多反应监测(MRM)进行检测。结果表明:13个功能成分在各自的线性范围内线性关系良好,相关系数R^(2)均大于0.99,该方法的检测限(LOD)和定量限(LOQ)分别为1.0~20.0μg/kg、2.0~50.0μg/kg,平均加标回收率在89.10%~109.51%范围内。LC-MS/MS法测定毕氏海蓬子功能成分快速灵敏,准确可靠,可用于毕氏海蓬子中功能成分的检测。The contents of 13 functional constituents such as gallocatechin,epigallocatechin,cryptochlorogenic acid,chlorogenic acid,neochlorogenic acid,catechin,epicatechin,isochlorogenic acid B,rutin,isoquercitrin,melatonin,kaempferol-3-O-rutinoside and quercitrin in Salicornia bigelovii were determined by UPLC-MS/MS.The samples were extracted with methanol,and then filtered through 0.2μm membranes before using the UPLC-MS/MS system for determination.The separation was carried out on C_(18)(100 mm×2.1 mm,1.7μm)with a mobile phase consisting of 0.1%formic acid-methanol at the flow rate of 0.3 mL/min.The column temperature was(35±5)℃.The salicornia bigeloviis were determined in the electrospray ionization mode and multiple reaction monitoring(MRM)mode.The results showed good linear relationships for all analytes in their respective ranges,with their correlation coefficients(R^(2))all above 0.99.The limits of detection(LOD)and the limits of quantifiction(LOQ)of the method were 1.0—20.0μg/kg and 2.0—50.0μg/kg,respectively.The recoveries of 13 functional constituents were in the range of 89.10%-109.51%.UPLC-MS/MS method is rapid,sensitive,accurate and reliable,so it can be applied to the determination of functional constituents in Salicornia bigelovii.

关 键 词：毕氏海蓬子 液相色谱-串级质谱 多功能成分检测 

分 类 号：R284.1[医药卫生—中药学]