Establishment of transposase-assisted low-input m^(6)A sequencing technique  

作　　者：Tao Chen Yan Li Dong-Zhao Ma Zhang Zhang Jian-Fei Xi Guan-Zheng Luo 

机构地区：[1]MOE Key Laboratory of Gene Function and Regulation,Guangdong Province Key Laboratory of Pharmaceutical Functional Genes,State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China

出　　处：《Journal of Genetics and Genomics》2021年第11期1036-1039,共4页遗传学报（英文版）

基　　金：the Ministry of Science and Technology of China(National Science and Technology Major Project,2019YFA0802203,2018YFA0109100);National Natural Science Foundation of China(31922015,31870808,91753129);Natural Science Foundation of Guangdong Province(2018B030306044,2019A1515110099);China Postdoctoral Science Foundation(2020M672949 and 2019M653164).

摘　　要：RNA modification has been recognized as a pivotal regulator that affects gene expression in a post-transcriptional manner.To date,more than 150 distinct types of RNA modification have been identi-fied,among which N6-methyladenosine(m6 A)is the most abundant and best-characterized internal modification in mRNAs(Boccaletto et al.,2018).Benefit from the fast development of innovative technologies,the prevalence of m6 A has been reported and confirmed repeatedly in diverse organisms.Recent studies reveal that aberrant m6 A modification is associated with various disease progressions such as carcinogenesis and tumorigenesis by disturbing the expression of oncogenes and tumor suppressor genes(Tuncel and Kalkan,2019;Zhao et al.,2020).The m6 A methyltransferase METTL3 is found to be overexpressed in acute myeloid leukemia(AML)(Barbieri et al.,2017;Vu et al.,2017),which consequently elevates the m6 A level of B cell lymphoma 2(BCL2)transcript and promotes the translation effi-ciency。

关 键 词：acute SUPPRESSOR 

分 类 号：Q75[生物学—分子生物学]