染色体缩合调控子2对食管癌小鼠细胞增殖的影响及其机制研究  

The effect of regulator of chromosome condensation 2 on the cell proliferation in esophageal cancer mouse models and its mechanisms

作　　者：陈民彪[1] 黄明芳[1] 廖绪强[1] 蔡仁中[1] Min-biao Chen;Ming-fang Huang;Xu-qiang Liao;Ren-zhong Cai(Department of Thoracic Surgery,Hainan General Hospital,Haikou,Hainan 570311,China)

机构地区：[1]海南省人民医院胸外科,海南海口570311

出　　处：《中国现代医学杂志》2021年第24期19-24,共6页China Journal of Modern Medicine

基　　金：海南省卫生计生行业科研项目(No:18A200050)。

摘　　要：目的探讨染色体缩合调控子2(RCC2)对食管癌小鼠细胞增殖的影响及其作用机制。方法提取食管癌小鼠食管组织及原代细胞,分组进行转染,采用CCK-8检测细胞增殖水平,RT-PCR和Western blotting检测RCC2、SOX2及增殖细胞核抗原(PCNA)、锌指蛋白(Snail)的表达,荧光素酶实验分析RCC2与SOX2的靶向关系。结果RCC2在食管癌组织中表达上调(P<0.05);过表达RCC2后食管癌小鼠细胞增殖水平升高,而抑制RCC2表达后细胞增殖水平降低(P<0.05);过表达RCC2后细胞中SOX2和SOX2靶基因PCNA、Snail相对表达量升高(P<0.05),而抑制RCC2表达后SOX2、PCNA、Snail相对表达量降低(P<0.05);荧光素酶实验结果显示RCC2与SOX2存在靶向调控关系。结论RCC2通过上调SOX2及其靶基因表达,提高食管癌小鼠细胞增殖能力,促进肿瘤细胞的发生、发展。Objective To explore the effect of regulator of chromosome condensation 2(RCC2)on the cell proliferation in mouse models with esophageal cancer and the underlying mechanisms.Methods Esophageal tissues and primary cells from esophageal cancer mouse models and normal mice were extracted.Gene transfection was applied to regulate the expression of RCC2.The cell proliferation was detected via cell counting kit-8(CCK-8),the expression of RCC2,sex-determining region Y-box 2(SOX2),proliferating cell nuclear antigen(PCNA),and zinc finger protein SnaiⅠwas determined via RT-PCR and Western blot,and the targeting relationship between RCC2 and SOX2 was analyzed via luciferase assay.Results The expression of RCC2 was upregulated in the esophageal cancer tissues(P<0.05).The cell proliferation was increased after the overexpression of RCC2(P<0.05),and was decreased after the inhibition of RCC2 expression(P<0.05).Following the overexpression of RCC2,the levels of SOX2 and its target genes,PCNA and SnaiⅠ,were significantly increased(P<0.05),while the levels of SOX2,PCNA and SnaiⅠwere significantly reduced after the inhibition of RCC2 expression(P<0.05).Luciferase assays showed that there was a targeting relationship between RCC2 and SOX2.Conclusions RCC2 can enhance the cell proliferation in esophageal cancer mouse models by up-regulating the expression of SOX2 and its target genes,thereby further promoting the tumor formation and progression.

关 键 词：食管癌 细胞增殖 染色体缩合调控子2 性别决定区Y框蛋白2 小鼠 

分 类 号：R735.1[医药卫生—肿瘤]