锌指蛋白582与老年宫颈癌患者放疗敏感性的相关性  

Correlation between zinc finger protein 582 gene methylation and radiosensitivity in elderly patients with cervical cancer

作　　者：祁小丽[1] 开丽曼·阿布都巴热 热伊拉·麦买提伊敏[1] 普拉提·努尔别克[1] QI Xiao-li;Kailiman ABUDUBARE;Reyila MAIMAITI YIMIN;Pulati NUERBIEKE(Radiotherapy Center,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang Uygur Autonomous Region 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院放疗中心,新疆维吾尔自治区乌鲁木齐830001

出　　处：《中华实用诊断与治疗杂志》2021年第12期1229-1232,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：新疆维吾尔自治区自然科学基金项目(2017D01C149)。

摘　　要：目的观察老年宫颈癌患者血清锌指蛋白(zinc finger protein,ZNF)582表达及甲基化程度,探讨其与老年宫颈癌患者放疗敏感性的关系。方法237例老年宫颈癌患者均行标准根治性放疗联合同步增敏化疗方案治疗,治疗期间出现严重化疗不良反应者停止增敏化疗,行单纯根治性标准放疗方案治疗。放疗完成后根据疗效分为放疗敏感组207例(完全缓解+部分缓解)和放疗耐受组30例(疾病稳定+疾病进展)。比较2组年龄、体质量指数、肿瘤直径、FIGO分期、病理类型、淋巴结转移及完成同步增敏化疗比率等临床资料;采用实时荧光定量PCR法检测2组患者放疗前血清ZNF582 mRNA相对表达量;检测2组患者放疗前血清ZNF582甲基化阳性率;采用多因素logistic回归分析老年宫颈癌患者发生放疗耐受的影响因素。结果放疗期间放疗敏感组35例、放疗耐受组11例发生化疗严重不良反应停止增敏化疗,采用单纯放疗。放疗敏感组FIGO分期Ⅰ~Ⅱ期(36.23%)、完成同步增敏化疗比率(83.09%)均高于放疗耐受组(16.67%、63.33%)(χ^(2)=4.485,P=0.034;χ^(2)=6.539,P=0.011);2组年龄、体质量指数、肿瘤直径、浸润深度、病理类型、人乳头瘤病毒阳性、淋巴结转移比率比较差异均无统计学意义(P>0.05);放疗敏感组血清ZNF582 mRNA相对表达量(0.99±0.24)高于放疗耐受组(0.78±0.22)(t=4.973,P<0.001),ZNF582甲基化阳性率(58.45%)低于放疗耐受组(90.00%)(t=11.119,P<0.001);血清ZNF582 mRNA相对表达量(OR=0.012,95%CI:0.008~0.397,P<0.001)、ZNF582甲基化(OR=7.704,95%CI:2.141~27.731,P=0.002)是老年宫颈癌患者发生放疗耐受的影响因素。结论老年宫颈癌患者放疗敏感者FIGO分期早,放疗联合同步增敏化疗可提高放疗疗效;血清ZNF582低表达及甲基化是老年宫颈癌患者发生放疗耐受的危险因素。Objective To observe the expression of serum zinc finger protein 582(ZNF582)and degree of methylation,and to explore the relationship between ZNF582 gene methylation and radiosensitivity in elderly patients with cervical cancer.Methods Totally 237 elderly patients with cervical cancer received radical standardized radiotherapy combined with concurrent sensitization chemotherapy.Those with serious adverse reactions to chemotherapy during treatment were terminated sensitization chemotherapy and received radical standardized radiotherapy alone.After the completion of radiotherapy,237 patients were divided into 207 patients with complete remission+partial remission(sensitive group)and 30 patients with stable disease+disease progression(tolerant group)according to the curative effect.The clinical data as age,body mass index,tumor diameter,FIGO stage,pathological type,lymph node metastasis and completion rate of concurrent sensitization chemotherapy were compared between two groups.Real-time fluorescence quantitative PCR was used to detect the relative expression of serum ZNF582 mRNA in two groups before radiotherapy.The positive rate of ZNF582 methylation was detected in two groups before radiotherapy.Multivariate logistic regression analysis was done to investigate the influencing factors of radiotherapy tolerance in elderly patients with cervical cancer.Results During chemothrapy,35 cases in sensitive group and 11 cases in tolerant group with severe adverse reactions to chemotherapy were terminated sensitization chemotherapy and received radiotherapy alone.The percentage of FIGO stageⅠtoⅡand completion rate of concurrent sensitization chemotherapy were higher in sensitive group(36.23%,83.09%)than those in tolerant group(16.67%,63.33%)(χ^(2)=4.485,P=0.034;χ^(2)=6.539,P=0.011),and there were no significant differences in age,body mass index,tumor diameter,depth of invasion,pathological type,HPV positive rate,and lymph node metastasis ratio between two groups(P>0.05).The relative expression of ZNF582 mRNA was hig

关 键 词：宫颈癌 老年 锌指蛋白582 甲基化 标准放疗 同步增敏化疗 

分 类 号：R737.33[医药卫生—肿瘤] R730.55[医药卫生—临床医学]