sigma-1受体对口腔鳞癌细胞增殖、迁移和侵袭影响的实验研究  

Experimental study on the effect of sigma-1 receptor on proliferation,migration and invasion of oral squamous cell carcinoma

作　　者：张红丽 徐小立 戴永铮 应松成 蒋勇[1,4] Zhang Hongli;Xu Xiaoli;Dai Yongzheng(Stomatological College of Anhui Medical University, The Affiliated Stomatological Hospital of Anhui Medical University, Key Laboratory of Oral Diseases Reasearch of Anhui Province, Hefei 230032;Dept of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032;Dept of General Dentistry, Hefei Stomatological Hospital, Hefei 230001)

机构地区：[1]安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究重点实验室,合肥230032 [2]安徽医科大学基础医学院免疫学教研室,合肥230032 [3]合肥市口腔医院综合科,合肥230001 [4]安徽医科大学第四附属医院口腔科,合肥230000

出　　处：《安徽医科大学学报》2021年第12期1908-1913,1919,共7页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1508085MH187)。

摘　　要：目的探究sigma-1受体在口腔鳞状细胞癌(OSCC)组织和细胞系中的表达,并通过特异性siRNA转染降低sigma-1受体在OSCC细胞中的表达,检测对其生物学行为的影响,并初步分析其可能的机制。方法免疫组化法检测人OSCC组织中sigma-1受体的表达。Western blot和免疫荧光法检测人OSCC细胞系中sigma-1受体的表达。利用GEPIA数据库分析sigma-1受体表达水平与OSCC患者预后的相关性。通过转染特异性siRNA下调sigma-1受体表达,CCK-8和Transwell迁移侵袭实验检测转染后OSCC细胞的增殖、迁移和侵袭,qRT-PCR和Western blot检测转染后电压门控钠离子通道亚型Nav1.5在OSCC细胞中的表达水平。结果免疫组化分析结果显示sigma-1受体在OSCC组织中较正常组织为高表达。Western blot检测结果显示,sigma-1受体在4种OSCC细胞系中均有表达。GEPIA数据库分析结果显示,sigma-1受体的表达水平与OSCC患者的总体生存率呈负相关。通过细胞免疫荧光实验结果显示sigma-1受体在CAL-27细胞中主要定位在质膜上。CCK-8分析结果显示sigma-1受体通过siRNA下调表达后降低了CAL-27细胞的增殖。Transwell分析结果显示降低sigma-1受体表达可显著抑制CAL-27细胞的迁移和侵袭。此外,下调sigma-1受体表达后Nav1.5的表达水平被抑制。结论sigma-1受体参与OSCC的发生发展,促进了OSCC细胞的增殖、迁移和侵袭,并可能通过调节Nav1.5离子通道参与OSCC的进展。Objective To investigate the expression of sigma-1 receptor in oral squamous cell carcinoma(OSCC)tissues and cell lines,reduce the expression of sigma-1 receptor in OSCC cells by transfection with specific siRNA,detect the effect on their biological behavior,and preliminarily analyze the possible mechanism.Methods The expression of sigma-1 receptor in human OSCC tissues were detected by immunohistochemistry.Western blot and immunofluorescence were used to detect the expression of sigma-1 receptor in human OSCC cell lines.GEPIA database was used to analyze the correlation between sigma-1 receptor expression level and prognosis of patients with OSCC. The expression of sigma-1 receptor was down-regulated by transfection of specific siRNA. Then the proliferation, migration and invasion of OSCC cells after transfection were detected by CCK-8 and Transwell assay, and the expression level of voltage-gated sodium ion channels subtype Nav1.5 in OSCC cells was detected by qRT-PCR and Western blot. Results Immunohistochemical analysis showed that the expression of sigma-1 receptor was higher in OSCC tissues than that in normal tissues. Western blot revealed that the sigma-1 receptor was expressed in all four OSCC cell lines. The results of GEPIA database analysis showed that the expression level of sigma-1 receptor was negatively correlated with the overall survival rate of patients with OSCC. Immunocytochemistry and confocal microscopy showed that the sigma-1 receptor was mainly localized in the plasma membrane in CAL-27 cells. CCK-8 analysis showed that the down-regulated expression of sigma-1 receptor reduced the proliferation of CAL-27 cells through its specific siRNA. Transwell analysis showed that the reduced expression of sigma-1 receptor significantly inhibited the migration and invasion of CAL-27 cells. In addition, down-regulation of sigma-1 receptor expression was found to inhibit the protein and mRNA expression levels of Nav1.5. Conclusion sigma-1 receptor participates in the occurrence and development of OSCC,

关 键 词：口腔鳞状细胞癌 sigma-1受体 电压门控钠离子通道 NAV1.5 

分 类 号：R739.85[医药卫生—肿瘤]