CCT4 suppression inhibits tumor growth in hepatocellular carcinoma by interacting with Cdc20  

作　　者：Feng Li Chun-Sheng Liu Ping Wu An-Sheng Ling Qi Pan Xiao-Ning Li 

机构地区：[1]Department of Gastroenterology,The First People’s Hospital of Anqing,Anqing,Anhui 246000,China [2]Department of Laboratory Medicine,Yijishan Hospital of Wannan Medical College,Wuhu,Anhui 241001,China [3]Department of Rehabilitation Medicine,The Sixth People’s Hospital of Anqing,Anqing,Anhui 246000,China

出　　处：《Chinese Medical Journal》2021年第22期2721-2729,共9页中华医学杂志（英文版）

基　　金：the Natural Science Foundation of Anhui Province(No.1908085QH325).

摘　　要：Background:The chaperonin containing t-complex(CCT)proteins play an important role in cell cycle-related protein degradation in yeast and mammals.The role of the chaperonin containing t-complex 4(CCT4),one subtype of CCT proteins,in the progress of hepatocellular carcinoma(HCC)was not fully elucidated.Here,we aimed to explore the mechanisms of CCT4 in HCC.Methods:In this study,we used the UALCAN platform to analyze the relationship between CCT4 and HCC,and the association of CCT4 with the overall survival(OS)of HCC patients was also analyzed.CCT4 expression in HCC tumor tissues and normal tissues was also determined by western blot(WB)assay.Lentivirus vector was used to knock down the CCT4 expression,and quantitative polymerase chain reaction and WB were used to determine the level of CCT4 in HCC cell lines.Cell counting kit-8(CCK-8)and 5-ethynyl-20-deoxyuridine(EdU)assays were used to detect the cell proliferation,and flow cytometry(FCM)was performed to evaluate the effect of CCT4 on the apoptosis of HCC cells.Co-immunoprecipitation(co-IP)assay and WB were used to explore the mechanisms of CCT4 regulating the growth of HCC.Data were calculated from at least three replicate experiments and expressed as mean±standard deviation.Student’s t test,paired t test,and Kaplan–Meier analysis were used to compare across different groups.Results:We found CCT4 was upregulated in HCC tissues compared with normal tissues,and its high expression was associated with poor prognosis(P<0.001).CCT4 was significantly increased in HCC tumor tissues compared with normal tissues(0.98±0.12 vs.0.23±0.05,t=7.73,P<0.001).After being transfected with CCT4 short-hairpin RNA(shRNA),CCT4 was decreased in mRNA level and protein level in both Huh7(mRNA level:0.41±0.07 vs.1.01±0.11,t=8.09,P=0.001;protein level:0.61±0.03 vs.0.93±0.07,t=7.19,P=0.002)and Hep3b cells(mRNA level:0.55±0.11 vs.1.04±0.15,t=4.51,P=0.011;protein level:0.64±0.10 vs.0.95±0.08,t=4.32,P=0.012).CCK8 assay indicated that CCT4 knockdown inhibited cell proliferation i

关 键 词：Hepatocellular carcinoma Chaperonin containing t-complex 4(CCT4) Cdc20 SECURIN Bim 

分 类 号：R735.7[医药卫生—肿瘤]