肾癌血管新生与肿瘤干细胞的相关性探索  

Relevance of renal cell carcinoma angiogenesis and tumor stem cells

作　　者：王璐[1] 付博 赵丹凤 杨光[2] 徐万海[1] Lu Wang;Bo Fu;Danfeng Zhao;Guang Yang;Wanhai Xu(Department of Urology,the Fourth Hospital of Harbin Medical University,Harbin,150001;Department of Neurosurgery,the First Affiliated Hospital of Harbin Medical University,Harbin 150001)

机构地区：[1]哈尔滨医科大学附属第四医院泌尿外科,哈尔滨150001 [2]哈尔滨医科大学附属第一医院神经外科,哈尔滨150001

出　　处：《中华医学杂志》2021年第46期3804-3808,共5页National Medical Journal of China

基　　金：国家自然科学基金(U20A20385);黑龙江省自然科学基金(YQ20202H022,YQ2020H014);黑龙江省博士后科研启动基金(21042190053)。

摘　　要：目的探索肿瘤血管新生与肾癌干细胞的相关性。方法应用细胞原代培养方法提取分离在哈尔滨医科大学附属医院泌尿外科接受手术的肾透明细胞癌患者的肾癌干细胞(RCSCs),随后利用qRT-PCR和Western印迹方法分别检测不同微血管密度(MVD)肾癌组织来源的肾癌干细胞标志物CD105、Sox2基因和蛋白表达水平。利用TCGA数据库数据,分析肿瘤血管新生标志物与肿瘤干细胞调控基因相关性。结果高MVD肾癌组织来源的RCSCs中干细胞标志物CD105和Sox2基因分别升高(2.34±1.77)倍和(3.92±1.41)倍(P_(CD105)<0.01,P_(Sox2)<0.05),蛋白水平升高(5.12±3.31)倍和(4.90±3.30)倍(P_(CD105)<0.05,P_(Sox2)<0.01)。高达30%的干细胞促“干性”调控基因与血管新生基因CD31/PECAM1、KDR存在正相关关系,并且有64个基因同时与CD31/PECAM1和KDR基因存在强正相关关系(r>0.6,P<0.05)。结论肾癌高微血管密度与肾癌干细胞存在强相关性。Objective To investigate the relationship between tumor angiogenesis and renal cancer stem cells.Methods The primary cell culture method was used to extract and isolate RCSCs,and then qRT-PCR and Western blot methods were used to detect the expression levels of the kidney cancer stem cell markers CD105 and Sox2 genes and proteins from different MVD kidney cancer tissues.Using TCGA database,analyze the correlation between tumor angiogenesis markers and tumor stem cell regulatory genes.Results The stem cell markers CD105 and Sox2 genes in RCSCs derived from high MVD kidney cancer tissues were respectively increased by(2.34±1.77)times and(3.92±1.41)times(P_(CD105)<0.01,P_(Sox2)<0.05)and protein levels were increased by(5.12±3.31)times and(4.90±3.30)times(P_(CD105)<0.05,P_(Sox2)<0.01).Meanwhile,up to 30%of stem cell promoting stemness regulatory genes are positively correlated with angiogenesis genes CD31/PECAM1 and KDR,and 64 genes are also strongly positively correlated with CD31/PECAM1 and KDR genes.Conclusion The high microvessel density of kidney cancer is strongly correlated with the existence of renal carcinoma stem cells.

关 键 词：肾肿瘤 癌 肾细胞 血管新生 微血管密度 肾癌干细胞 

分 类 号：R737.11[医药卫生—肿瘤]