长链非编码RNA(lncRNA)核仁小RNA宿主基因7(SNHG7)对葡萄膜黑色素瘤癌细胞增殖和侵袭力的影响  

Effects of long non-coding RNA small nucleolar RNA host gene 7 on the proliferation and invasiveness of uveal melanoma cells

作　　者：张菡[1] 高彦[1] 薛春丽[1] 陈涛[1] ZHANG Han;GAO Yan;XUE Chunli;CHEN Tao(Department of Ophthalmology,Zhengzhou People’s Hospital,Zhengzhou 450001,Henan Province,China)

机构地区：[1]郑州人民医院眼科,河南省郑州市450001

出　　处：《眼科新进展》2021年第12期1112-1115,共4页Recent Advances in Ophthalmology

基　　金：河南省科技攻关计划(编号LHGJ20190238)。

摘　　要：目的探讨长链非编码RNA(lncRNA)核仁小RNA宿主基因7(SNHG7)对葡萄膜黑色素瘤癌细胞增殖和侵袭力的影响。方法选取2011年8月至2019年8月在我院行手术治疗的葡萄膜黑色素瘤患者71例71眼作为葡萄膜黑色素瘤组,另选取因外伤摘除且葡萄膜完整、眼球正常的患者40例40眼作为正常组。两组患者性别、年龄、患眼眼别差异均无统计学意义(均为P>0.05)。采用实时荧光定量PCR检测两组患者眼球组织中SNHG7表达。取M23细胞进行培养,将对数生长期细胞分成3组,SNHG7干扰组转染SNHG7干扰序列;阴性对照组转染阴性对照序列;空白组不作任何处理。采用实时荧光定量PCR检测各组细胞中SNHG7表达,CCK-8法检测转染后细胞增殖活性,Transwell法检测细胞侵袭力。结果葡萄膜黑色素瘤组患者眼球组织中SNHG7的相对表达量为2.05±0.16,正常组患者眼球组织中SNHG7的相对表达量为1.01±0.07,两组差异有统计学意义(t=39.461,P<0.001)。葡萄膜黑色素瘤组在发生巩膜浸润和眼外生长的患者眼球组织中SNHG7相对表达量均较无巩膜浸润和无眼外生长的患者高,差异均有统计学意义(均为P<0.05)。转染后继续培养48 h,SNHG7干扰组细胞SNHG7相对表达量(0.27±0.09)明显低于阴性对照组(1.01±0.07)和空白组(1.03±0.09)(均为P<0.001)。与阴性对照组和空白组相比,SNHG7干扰组细胞转染后24 h、48 h、72 h和96 h时光密度均降低,差异均有统计学意义(均为P<0.05)。转染后48 h,SNHG7干扰组侵袭细胞数(89.77±9.82)个显著低于阴性对照组(133.14±7.54)个和空白组(137.09±10.05)个(均为P<0.001)。结论葡萄膜黑色素瘤患者眼球组织中SNHG7呈高表达,且SNHG7高表达与肿瘤组织巩膜浸润和眼外生长有关,下调SNHG7基因表达可阻碍M23细胞增殖和侵袭。Objective To investigate the effect of long non-coding RNA(lncRNA)small nucleolar RNA host gene 7(SNHG7)on the proliferation and invasiveness of uveal melanoma cells.Methods A total of 71 eyes of 71 patients with uveal melanoma(UM)who underwent surgical treatment in our hospital from August 2011 to August 2019 were selected as the UM group.In addition,40 eyes of 40 patients with intact uvea and normal eyeballs were selected as the normal group.There were no statistically significant differences in gender,age,and eye type between the two groups(all P>0.05).The real-time quantitative polymerase chain reaction(PCR)was used to detect the expression of SNHG7 in the eyeball tissues of patients in the two groups.M23 cells were cultured,and those in the logarithmic growth phase were divided into 3 groups SNHG7 interference group(transfected with SNHG7 interference sequence),negative control group(transfected with negative control sequence),and blank group(no treatment).The real-time quantitative PCR was used to detect the expression of SNHG7 in cells,the CCK-8 method was used to detect the cell proliferation activity after transfection,and the Transwell method was used to detect the cell invasion.Results The relative expression of SNHG7 in the eyeball tissues of UM group was 2.05±0.16,while that of the normal group was 1.01±0.07.The difference between the two groups was statistically significant(t=39.461,P<0.001).In the UM group,the relative expression of SNHG7 in the eyeball tissues of patients with scleral infiltration and extraocular growth was higher than that of patients without scleral infiltration or extraocular growth,and the difference between the two groups was statistically significant(both P<0.05).After cells were transfected and cultured for 48 h,the relative expression of SNHG7 in the SNHG7 interference group(0.27±0.09)was significantly lower than that in the negative control group(1.01±0.07)and the blank group(1.03±0.09)(both P<0.001).Compared with the negative control group and the blank group,the op

关 键 词：葡萄膜黑色素瘤 核仁小RNA宿主基因7 临床病理学 细胞增殖 细胞侵袭 

分 类 号：R773[医药卫生—眼科]